Softening rate of the Chilean strawberry (Fragaria chiloensis) fruit reflects the expression of polygalacturonase and pectate lyase genes
Softening rate of the Chilean strawberry (Fragaria chiloensis) fruit reflects the expression of polygalacturonase and pectate lyase genes
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DOI：
10.1016/j.postharvbio.2008.01.018
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发表时间：
2008-08-01
影响因子：
7
通讯作者：
Moya-Leon, Maria Alejandra
中科院分区：
文献类型：
--
作者：
Figueroa, Carlos R.;Pimentel, Paula;Moya-Leon, Maria Alejandra
智利草莓（Fragaria chiloensis）以其优良的果实品质而闻名，是商品草莓（Fragaria x ananassa）的野生亲本之一。然而，智利草莓的果实比F。x ananassa，这是一个可以影响其保质期并降低其保存质量的因素。在不同的发育和成熟时间收集这两个物种的果实，并根据重量和颜色分为四个阶段：阶段1和2对应于小，未成熟和硬的果实，而阶段3和4对应于颜色断路器和成熟的果实。从F. Chiloensis的FcPG 1和FcPL 1分别编码多聚半乳糖醛酸酶（PG）和果胶酸裂解酶（PL）蛋白。In F. chiloensis中PG转录本在第2和第3阶段之间显著增加，而在F. x ananassa在相同的发展阶段。PL在F. chiloensis的转录水平在第2和第4阶段之间逐渐增加，而F. x ananassa中的F.智利人。这两个基因表达的增加与果实中观察到的硬度降低密切相关。F. Southern杂交分析表明，PG和PL基因在两种植物中均属于多基因家族。本研究强调了PG和PL在F软化过程中的参与。chiloensis果实，并给出了目标基因，可用于帮助草莓育种计划的指示。(C)2008 Elsevier B.V.保留所有权利。
The Chilean strawberry (Fragaria chiloensis) is noted for its good fruit quality characters and is one of the wild parents of the commercial strawberry (Fragaria x ananassa). However, the fruit of the Chilean strawberry shows a higher level of softening than that of F. x ananassa, a factor that can affect its shelf-life and reduce its keeping quality. Fruit of both species were collected at different developmental and ripening times, and classified into four stages according to weight and color: stages 1 and 2 correspond to small, unripe and hard fruit, while stages 3 and 4 correspond to color-breaker and ripening fruit. Two full-length cDNAs were isolated from F. chiloensis, FcPG1 and FcPL1 encoding polygalacturonase (PG) and pectate lyase (PL) proteins, respectively. In F. chiloensis a considerable increase in PG transcripts was observed between stages 2 and 3, while a lower expression level was observed in F. x ananassa in the same developing stages. The expression pattern of PL in F. chiloensis revealed a gradual increment in the transcript level between stages 2 and 4, with a higher increment between stages 2 and 3 in F. x ananassa than in F. chiloensis. The increase in expression of both genes correlated well with the decrease in firmness observed in the fruit. The faster softening observed in F. chiloensis may result from the earlier and higher level expression of PG. In addition, Southern blot analyses showed that PG and PL genes belong to a multigene family in both species. This study highlights the participation of PG and PL during softening of F. chiloensis fruit, and gives an indication of target genes that can be used to help strawberry breeding programmes. (C) 2008 Elsevier B.V. All rights reserved.