Liquid-like phases preorder peptides for assembly
Liquid-like phases preorder peptides for assembly
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类液相预排序肽进行组装
DOI：
10.1002/syst.202000007
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Lynn, David G
中科院分区：
文献类型：
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作者：
2. Rengifo, Rolando F;Sementilli, Anthony;Kim, Youngsun;Liang, Chen;Li, Noel Xiang’An;Mehta, Anil K;Lynn, David G
控制超分子组装仍然是材料科学和合成生物学的重大挑战。生物聚合物通过涉及动态中间溶质富集相的两步成核过程组织成多分子结构。在这里，我们提出了与阿尔茨海默病Aβ肽的同源物形成的亚稳相的光谱分析，揭示了单一β折叠的不同群体。这种液态粒子相的有序度在片层范围和单个传播核的选择中都是显着的。所得的原纤维种子在溶液中不太稳定，并协同转化为另一种原纤维。这种肽的构象动力学提供了一种控制健康和疾病中多态性淀粉样蛋白组装体范围的机制模型。
Controlling supramolecular assembly remains a major challenge for materials science and synthetic biology. Biopolymers organize into multimolecular architectures via two‐step nucleation processes involving dynamic intermediate solute‐rich phases. Here we present spectroscopic analyses of metastable phases formed with a congener of the Alzheimer's disease Aβ peptide that reveals diverse populations of single β‐sheets. The degree of order in this liquid‐like particle phase is remarkable both in the range of sheets and the selection of a single propagating nucleus. The resulting fibril seed is less stable in solution and cooperatively transforms into another fibril. The conformational dynamics of this peptide provide a mechanistic model for controlling the range of polymorphic amyloid assemblies in health and disease.