Mitochondria Synthesize Melatonin to Ameliorate Its Function and Improve Mice Oocyte's Quality under in Vitro Conditions.
Mitochondria Synthesize Melatonin to Ameliorate Its Function and Improve Mice Oocyte's Quality under in Vitro Conditions.
复制标题
DOI：
10.3390/ijms17060939
复制
发表时间：
2016-06-14
影响因子：
5.6
通讯作者：
Liu G
中科院分区：
文献类型：
--
作者：
He C;Wang J;Zhang Z;Yang M;Li Y;Tian X;Ma T;Tao J;Zhu K;Song Y;Ji P;Liu G
卵母细胞体外成熟的生理学仍然难以捉摸。一般来说，卵母细胞在体外条件下的成熟率非常低。在目前的研究中，我们发现褪黑激素促进卵母细胞的成熟，其中线粒体发挥着关键作用。经查明； (1)线粒体是卵母细胞合成褪黑激素的主要场所，在成熟过程中合成大量褪黑激素； (2) 褪黑素通过增加卵母细胞中线粒体DNA拷贝、线粒体膜电位(ΔΨm)以及线粒体分布和ATP产生来改善线粒体功能； (3)减数分裂纺锤体组装增强； (4) 褪黑激素减少ROS的产生并抑制8-oxodG的形成，从而保护潜在的DNA突变免受氧化损伤。结果，褪黑激素提高了卵母细胞的质量，显着加速了IVF胚胎的发育能力。这些结果提供了有关卵母细胞成熟生理学的新知识，特别是在体外条件下。
The physiology of oocyte in vitro maturation remains elusive. Generally, the oocytes have a very low maturation rate under in vitro conditions. In the current study, we found that melatonin promotes the maturation of oocytes in which mitochondria play a pivotal role. It was identified that; (1) mitochondria are the major sites for melatonin synthesis in oocytes and they synthesize large amounts of melatonin during their maturation; (2) melatonin improves mitochondrial function by increased mtDNA copy, mitochondrial membrane potential (ΔΨm) and mitochondrial distribution and ATP production in oocytes; (3) the meiotic spindle assembly is enhanced; (4) melatonin reduces ROS production and inhibits 8-oxodG formation, thereby protecting potential DNA mutation from oxidative damage. As a result, melatonin improves the quality of oocytes, significantly accelerates the developmental ability of IVF embryo. The results provide novel knowledge on the physiology of oocyte’s maturation, especially under in vitro conditions.