Incorporation of glucose oxidase into Langmuir-Blodgett films based on Prussian blue applied to amperometric glucose biosensor
Incorporation of glucose oxidase into Langmuir-Blodgett films based on Prussian blue applied to amperometric glucose biosensor
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DOI：
10.1021/la063175g
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发表时间：
2007-04-10
期刊：
影响因子：
3.9
通讯作者：
Izumi, Mitsuru
中科院分区：
文献类型：
--
作者：
Ohnuki, Hitoshi;Saiki, Takafumi;Izumi, Mitsuru
葡萄糖氧化酶 (GOx) 固定在由十八烷基三甲基铵 (ODTA) 和纳米普鲁士蓝 (PB) 簇组成的有机-无机 Langmuir-Bldogett (LB) 膜中。制备并测试了基于LB膜的电流型葡萄糖生物传感器。结果发现，传感器在施加电压为 0.0 V（相对于 Ag/AgCl）时表现出清晰的响应电流。电流密度与葡萄糖浓度的线性关系在浓度低于 15 mmol/L 时得到证实。这是有机-无机杂化LB膜生物传感器功能的首次观察。在极低电位下工作的葡萄糖传感器的成功制备表明，LB 膜中吸附的 PB 簇可以作为过氧化氢电化学还原的电催化剂，过氧化氢是酶促反应序列的最终产物。观察到的低潜在适用性估计会抑制抗坏血酸、尿酸和对乙酰氨基酚等干扰物的反应。还发现带正电荷的 ODTA(+) 与 GOx 和 PB 的吸附物质之间的静电相互作用在 LB 膜中提供了稳定的吸附状态。这种稳定的固定有助于在本发明的 ODTA/PB/GOx LB 膜中观察到稳定的电流响应电流。
Glucose oxidase (GOx) was immobilized in the organic-inorganic Langmuir-Bldogett (LB) films consisting of octadecyltrimethylammonium (ODTA) and nanosized Prussian blue (PB) clusters. The amperometric glucose biosensors based on the LB films were fabricated and tested. It was found that the sensors exhibited a clear response current under an applied voltage of 0.0 V (vs Ag/AgCl). The linearity of current density versus glucose concentration was confirmed below 15 mmol/L concentration. This is the first observation of biosensor function of the hybrid organic-inorganic LB films. The successful preparation of glucose sensors operating at the very low potential indicates that the adsorbed PB clusters in the LB films act as an electrocatalyst for the electrochemical reduction of hydrogen peroxide, which is the final product of the enzymatic reaction sequence. The observed low potential applicability is estimated to inhibit the responses of interferants such as ascorbic acid, uric acid, and acetominophen. It was also found that an electrostatic interaction between positively charged ODTA(+) and the adsorbed species of both GOx and PB provided a stabilized adsorption state in the LB films. Such stable immobilization contributes to the steady amperometric response current observed in the present ODTA/PB/GOx LB films.