Delimitation and properties of DNA sequence: required for the regulated expression of human interferon-β gene
Delimitation and properties of DNA sequence: required for the regulated expression of human interferon-β gene
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DNA序列的界定和特性：人干扰素-β基因的调控表达所需
DOI：
10.1016/s0092-8674(85)80022-2
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发表时间：
1985
期刊：
影响因子：
64.5
通讯作者：
T. Taniguchi
中科院分区：
文献类型：
--
作者：
T. Fujita;S. Ohno;H. Yasumitsu;T. Taniguchi
人干扰素-β（IFN-β）基因在其5′-侧翼区域内含有介导病毒诱导的mRNA转录激活的序列。构建了包括5′端缺失突变体在内的一系列IFN-β基因突变体，并将其导入小鼠L929细胞。对这些突变基因的表达研究表明：帽位点-40上游而非下游的序列负责基因的病毒诱导;支持最大诱导水平所需的DNA序列的上游边界位于帽位点-117和-105之间;并且该上游序列显示出类似于增强子元件的性质，因为它在任一方向上起作用，与帽位点的距离具有一定范围。在该序列中，我们注意到存在重复的六核苷酸（共有序列：），每个六核苷酸都可能在IFN-β基因的最大诱导中发挥作用。
The human interferon-β (IFN-β) gene contains sequences within its 5′-flanking region that mediate the virus-induced activation of mRNA transcription. A series of mutant IFN-β genes, including the 5′ deletion mutants, was constructed and introduced into mouse L929 cells. Expression study of those mutant genes demonstrates that: sequences upstream, but not downstream, of −40 from the cap site are responsible for the viral induction of the gene; that the upstream boundary of the DNA sequences required to support the maximum level of induction lies between −117 and −105 from the cap site; and that this upstream sequence shows a property similar to enhancer elements as it functions in either orientation with a latitude in distance from the cap site. Within this sequence, we note the presence of repetitious hexanucleotides (consensus:), each of which may play a role in the maximum induction of the IFN-β gene.
在用克隆的 cDNA 质粒转染的小鼠细胞中用聚 (rI.rC) 诱导人 β-干扰素合成。
DOI：
10.1073/pnas.79.14.4337
发表时间：
1982
影响因子：
11.1
作者：
Pitha,PM;Ciufo,DM;Kellum,M;Raj,NB;Reyes,GR;Hayward,GS
通讯作者：
Hayward,GS