EARLY MOUSE MOLAR ROOT DEVELOPMENT - CELLULAR-CHANGES AND DISTRIBUTION OF FIBRONECTIN, LAMININ AND TYPE-IV COLLAGEN
EARLY MOUSE MOLAR ROOT DEVELOPMENT - CELLULAR-CHANGES AND DISTRIBUTION OF FIBRONECTIN, LAMININ AND TYPE-IV COLLAGEN
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DOI：
10.1111/j.1432-0436.1985.tb00522.x
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发表时间：
1985-01-01
期刊：
影响因子：
2.9
通讯作者：
GRIMAUD, JA
中科院分区：
文献类型：
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作者：
ANDUJAR, MB;MAGLOIRE, H;GRIMAUD, JA
我们利用光镜和电子显微镜分析了小鼠磨牙根形成早期发生的上皮-间充质相互作用。描述了Hertwig的上皮鞘、髓质和滤泡间质细胞的形态学变化。Hertwig的上皮细胞失去了它们的立方体，变得扁平，显然与滤泡间质细胞混合在一起。在光镜和电镜下，利用免疫过氧化物酶技术定位纤维连接蛋白、层粘连蛋白和iv型胶原。这些似乎与发育中的牙根细胞分化和基质沉积密切相关。此外，在超微结构水平上检测细胞内免疫反应性。面对脱细胞牙骨质的牙周韧带部分细胞的粗糙内质网和核膜对层粘连蛋白和iv型胶原表现出特异性反应性。此外，这些牙周韧带细胞表达角蛋白，但不表达波形蛋白。我们的研究结果表明，尽管基底膜断裂和Hertwig上皮健康紊乱，Hertwig上皮细胞仍保持其合成层粘连蛋白和iv型胶原的能力，以及表达角蛋白丝的能力。因此，一些Hertwig上皮细胞保留在牙周韧带内，与滤泡间充质细胞混合。
We analyzed epithelial-mesenchymal interactions that occur during the early stages of the formation of mouse molar roots using light and electron microscopy. Morphological changes observed in the cells of Hertwig''s epithelial sheath, the pulp and the follicular mesenchyme are described. The Hertwig''s epithelial cells lose their cuboidal form and become flattened, apparently intermixing with the cells of the follicular mesenchyme. At the light- and electron-microscope levels, immunoperoxidase techniques were used to localize fibronectin, laminin and type-IV collagen. These appear to be closely associated with cell differentiation and matrix deposition in developing tooth roots. In addition, at the ultrastructural level, intracellular immunoreactivity was detected. The rough endoplasmic reticulum and nuclear envelope of some cells of the periodontal ligament facing the acellular cementum exhibited specific reactivity with laminin and type-IV collagen. Moreover, these periodontal ligament cells express keratin, but not vimentin, filaments. Our results demonstrate that Hertwig''s epithelial cells maintain their capacity to synthesize laminin and type-IV collagen, as well as to express keratin filaments, despite basement membrane fragmentation and the disorganization of Hertwig''s epithelial shealth. Thus, some Hertwig''s epithelial cells remain in the periodontal ligament intermixed with follicular mesenchyme clels.