Enhanced ALP activity of MG63 cells cultured on hydroxyapatite-poly(ethylene glycol) hydrogel composites prepared using EDTA-OH
Enhanced ALP activity of MG63 cells cultured on hydroxyapatite-poly(ethylene glycol) hydrogel composites prepared using EDTA-OH
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DOI：
10.1088/1748-6041/10/1/015025
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发表时间：
2015-02-01
影响因子：
4
通讯作者：
Taguchi, Tetsushi
中科院分区：
文献类型：
--
作者：
Ito, Temmei;Sasaki, Makoto;Taguchi, Tetsushi
为了获得羟基磷灰石（HAp）-聚乙二醇（PEG）复合材料，使用二琥珀酰亚胺基酒石酸酯交联四胺封端的PEG以获得PEG水凝胶。使用对Ca离子具有不同稳定常数的两种螯合剂（N-（2-羟乙基）乙二胺-N，N ′，N ′-三乙酸（EDTA-OH，8.14）和乙二胺-N，N，N ′，N ′-四乙酸（EDTA，10.96）），通过在90 ℃下加热含有螯合剂的磷酸钙溶液，磷酸钙沉积在PEG水凝胶内。X射线衍射分析表明沉积的磷酸钙为HAp。使用EDTA-OH沉积的HAp的结晶度与使用EDTA获得的结晶度相比低，但是使用EDTA-OH沉积在PEG水凝胶内的HAp的量高于使用EDTA沉积的量。与使用EDTA制备的HAp-PEG复合物相比，使用EDTA-OH制备的HAp-PEG复合物上粘附的人成骨细胞样MG-63细胞显著更多。此外，MG-63在使用EDTA-OH制备的HAp-PEG复合物上培养的碱性磷酸酶活性比在使用EDTA制备的HAp-PEG复合物上培养的碱性磷酸酶活性高4倍。因此，使用EDTA-OH制备的HAp-PEG复合物具有作为骨替代材料的潜力。
In order to obtain a hydroxyapatite (HAp)-poly(ethylene glycol) (PEG) composite, tetra amine-terminated PEG was crosslinked using disuccinimidyl tartrate to obtain a PEG hydrogel. Using two kinds of chelators with different stability constants for Ca ion (N-(2-hydroxyethyl) ethylenediamine-N, N', N'-triacetic acid (EDTA-OH, 8.14), and ethylenediamine-N, N, N', N'-tetraacetic acid (EDTA, 10.96)), calcium phosphate was deposited within PEG hydrogels by heating the chelator-containing calcium phosphate solution at 90 degrees C. X-ray diffraction analysis showed that the deposited calcium phosphate was HAp. The crystallinity of the HAp deposited using EDTA-OH was low compared with that obtained using EDTA, but the amount of HAp deposited within the PEG hydrogel using EDTA-OH was higher than that deposited using EDTA. Significantly more human osteoblast-like MG-63 cells adhered on the HAp-PEG composite prepared using EDTA-OH than on the HAp-PEG composites prepared using EDTA. Furthermore, the alkaline phosphatase activity of MG-63 cultured on the HAp-PEG composite prepared using EDTA-OH was four times higher than that on the HAp-PEG composite prepared using EDTA. Therefore, the HAp-PEG composite prepared using EDTA-OH has potential as a bone substitute material.