Comparative proteomics of short and tall glandular trichomes of Nicotiana tabacum reveals differential metabolic activities.
Comparative proteomics of short and tall glandular trichomes of Nicotiana tabacum reveals differential metabolic activities.
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DOI：
10.1021/pr5002548
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发表时间：
2014-06
影响因子：
4.4
通讯作者：
Adrienne Sallets;Maxime Beyaert;M. Boutry;A. Champagne
中科院分区：
文献类型：
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作者：
Adrienne Sallets;Maxime Beyaert;M. Boutry;A. Champagne
叶片腺毛（表皮毛）积极合成次生代谢产物，其中许多是植物防御的前线。在烟草中，已经发现了高的和短的腺毛。虽然前者已被广泛研究并与毛状体功能的经典图像相匹配，但短毛状体仍然相对不具特征。我们建立了一种基于Percoll密度梯度的离心分离程序，以获得纯化率为bb0 - 85%的高、短毛状体分离馏分。然后，我们研究了两种毛状体类型的蛋白质组，结合2D-LC分离色氨酸肽，并使用等压标记对一组461个蛋白质组进行相对和绝对定量。几乎整个导致二萜合成的途径都是在高毛状体中确定的。还发现了它们在角质层化合物合成中的关键作用。短腺毛的核糖体蛋白和酶如磷酸烯醇丙酮酸羧激酶和多酚氧化酶比高腺毛丰富。这些结果在关于短毛和长毛腺各自作用的几个假设的框架内进行了讨论。
Leaf glandular trichomes (epidermal hairs) actively synthesize secondary metabolites, many of which are the frontline of plant defense. In Nicotiana tabacum, tall and short glandular trichomes have been identified. While the former have been extensively studied and match the classic picture of trichome function, the short trichomes have remained relatively uncharacterized. We have set up a procedure based on centrifugation on Percoll density gradients to obtain separate tall and short trichome fractions purified to >85%. We then investigated the proteome of both trichome types combining 2D-LC fractionation of tryptic peptides and quantification of a set of 461 protein groups using isobaric tags for relative and absolute quantitation. Almost the entire pathway leading to the synthesis of diterpenes was identified in the tall trichomes. Indications for their key roles in the synthesis of cuticular compounds were also found. Concerning the short glandular trichomes, ribosomal proteins and enzymes such phosphoenolpyruvate carboxykinase and polyphenol oxidase were more abundant than in the tall glandular trichomes. These results are discussed in the frame of several hypotheses regarding the respective roles of short and long glandular trichomes.