THE HEPATOPANCREAS AND OVARY ARE THE SITES OF VITELLOGENIN SYNTHESIS IN FEMALE RED SWAMP CRAYFISH (PROCAMBARUS CLARKII (GIRARD, 1852)) (DECAPODA: ASTACOIDEA: CAMBARIDAE)
THE HEPATOPANCREAS AND OVARY ARE THE SITES OF VITELLOGENIN SYNTHESIS IN FEMALE RED SWAMP CRAYFISH (PROCAMBARUS CLARKII (GIRARD, 1852)) (DECAPODA: ASTACOIDEA: CAMBARIDAE)
复制标题
肝胰腺和卵巢是雌性红沼泽小龙虾（克氏原螯虾 (GIRARD, 1852)）卵黄原蛋白合成的场所（十足目：虾总科：螯虾科）
DOI：
10.1163/1937240x-00002459
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发表时间：
2016-09-01
影响因子：
1.1
通讯作者：
Liao, Xiang-Ru
中科院分区：
文献类型：
--
作者：
Guan, Zheng-Bing;Yin, Jun;Liao, Xiang-Ru
Vitellogenin (Vg) is the precursor of vitellin, the major yolk protein. The site of Vg synthesis in crustaceans remains controversial. This study investigated the synthesis site of Vg in female red swamp crayfish Procambarus clarkii (Girard, 1852). Results revealed that the mRNA of Vg was detected in the tissues of hepatopancreas of P. clarkii. The mRNA expression level of Vg increased in the previtellogenic stage and peaked in the vitellogenic stage in the hepatopancreas and ovary; the mRNA expression level of Vg in the hepatopancreas was approximately 20-25-fold of that of the ovary in the same ovarian stage. Eyestalk ablation could upregulate the mRNA expression level of Vg in both organs. Our RNAi experiment verified that the hepatopancreas and ovary are the Vg synthesis sites in female P. clarkii.
