THE CYTOLOGY OF DOPAMINERGIC AND NONDOPAMINERGIC NEURONS IN THE SUBSTANTIA NIGRA AND VENTRAL TEGMENTAL AREA OF THE RAT - A LIGHT-MICROSCOPIC AND ELECTRON-MICROSCOPIC STUDY
THE CYTOLOGY OF DOPAMINERGIC AND NONDOPAMINERGIC NEURONS IN THE SUBSTANTIA NIGRA AND VENTRAL TEGMENTAL AREA OF THE RAT - A LIGHT-MICROSCOPIC AND ELECTRON-MICROSCOPIC STUDY
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DOI：
10.1016/0306-4522(83)90007-6
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发表时间：
1983-01-01
期刊：
影响因子：
3.3
通讯作者：
PASKEVICH, PA
中科院分区：
文献类型：
--
作者：
DOMESICK, VB;STINUS, L;PASKEVICH, PA
在光镜和电镜下，多巴胺能细胞可通过细胞学特征，特别是尼氏物质的类型与非多巴胺能细胞区分开来。在黑质和腹侧被盖区，可以在尼氏制剂中鉴定出2种主要类型的细胞：深染色的嗜碱性细胞，具有大量的尼氏物质，以及浅染色的细胞，具有更半透明的细胞质。黑质和腹侧被盖区的嗜碱性细胞都是多巴胺能细胞。嗜碱性细胞和多巴胺能细胞的组织荧光和免疫组织化学方法绘制的地形分布之间有很好的相关性。通过脑内注射7-羟基多巴胺在多巴胺能通路中单侧破坏多巴胺能神经元后，在病变侧黑质和腹侧被盖区的嗜碱性细胞消失，而较浅染色的细胞似乎不受影响。在正常大鼠和单侧6-羟基多巴胺损伤的大鼠中，静脉注射[3 H]去甲肾上腺素用于多巴胺能神经元的特异性标记。在放射自显影的半薄切片，这样的标记只观察到在深染色，而不是在光染色的细胞，并在单侧6-羟基多巴胺病变的情况下，完全不存在的病变侧。电镜显示，嗜碱性多巴胺能细胞的大部分细胞质中密集地充满了游离核糖体，这些核糖体与粗面内质网的大的、组织良好的复合体相关联。浅色非多巴胺能细胞的细胞质中仅含有稀疏的游离核糖体和小的、分布广泛的粗面内质网聚集体。这两种细胞类型的大小和形状相似。
Dopaminergic cells can be distinguished from nondopaminergic cells, at both the light-microscopy and EM, by cytological features, and particularly by the pattern of Nissl substance. In both the substantia nigra and the ventral tegmental area, 2 main categories of cell types can be identified in Nissl preparations: dark-staining, basophilic cells with large masses of Nissl substance and light-staining cells with more translucent cytoplasm. Basophilic cells of both substantia nigra and ventral tegmental area are the dopaminergic cells. There is a good correlation between the topographic distribution of basophilic cells and that of dopaminergic cells mapped by both histofluorescence and immunohistochemical methods. After unilateral destruction of the dopaminergic neurons by intracerebral injection of 7-hydroxydopamine in the dopaminergic pathway, the basophilic cells in the substantia nigra and ventral tegmental area disappeared on the lesion side, while the lighter-staining cells appeared unaffected. In normal rats, and in rats with unilateral 6-hydroxydopamine lesions, i.v. injection of [3H]norepinephrine was used for specific labeling of dopaminergic neurons. In autoradiograms of semithin sections, such labeling was observed only in dark-staining and not in light-staining cells, and in cases of unilateral 6-hydroxydopamine lesions was totally absent on the lesion side. EM showed much of the cytoplasm of the basophilic dopaminergic cells to be densely filled with free ribosomes associated with large, well organized complexes of rough endoplasmic reticulum. The cytoplasm of the light, nondopaminergic cells contains only sparse free ribosomes and small, widely spaced aggregates of rough endoplasmic reticulum. Both cell types occur in a similar variety of size and shape.