Quantitative Trait Loci Associated with Physiological Traits for Heat Tolerance in Creeping Bentgrass
Quantitative Trait Loci Associated with Physiological Traits for Heat Tolerance in Creeping Bentgrass
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DOI：
10.2135/cropsci2015.07.0428
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发表时间：
2016-05-01
期刊：
影响因子：
2.3
通讯作者：
Huang, Bingru
中科院分区：
文献类型：
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作者：
Jespersen, David;Merewitz, Emily;Huang, Bingru
热胁迫相关数量性状位点（QTL）的鉴定对耐热种质资源的选择具有重要意义。本研究的目的是通过将匍匐弯草（Agrostis stolonifera L.）群体中与耐热性相关的主要生理性状与基因组特定区域的连锁图谱和耐热性遗传变异联系起来，鉴定QTL标记。在2年的田间试验（2008年和2009年）、温室和受控环境生长室中，评估了热胁迫条件下草坪视觉质量（TQ）评级、叶片叶绿素含量（CHL）、叶膜稳定性和冠层温度下降（CTD）的表型变化。表型评估证明了群体中耐热性的分离和变异，并允许基因组特定区域的性状关联。在两个亲本连锁图谱中发现了每个性状的数量性状位点，所有性状共有32个QTL。一些QTL区域与多个性状相关，如L93-10连锁图谱的2.2、4.1和4.2连锁组，7418-3连锁图谱的3.1和5.1连锁组。本研究中发现的数量性状位点可以在未来的研究中用于标记辅助选择，以提高匍匐弯草的耐热性或帮助阐明耐热性的调节机制。
Identification of heat-stress-related quantitative trait loci (QTL) is important for selecting heat-tolerant germplasm. The aim of this study was to identify QTL markers by associating major physiological traits related to heat tolerance to specific regions of the genome in a population of creeping bentgrass (Agrostis stolonifera L.) with linkage maps and genetic variability for heat tolerance. Phenotypic variation in visual turf quality (TQ) ratings, leaf chlorophyll content (CHL), leaf membrane stability, and canopy temperature depression (CTD) were evaluated under heat-stress conditions in 2-yr field trials (2008 and 2009), greenhouses, and controlled-environmental growth chambers. Phenotypic assessment demonstrated the segregation and variation of heat tolerance in the population and allowed for the association of traits in specific regions of the genome. Quantitative trait loci for every trait were found across the two parental linkage maps, with a total of 32 QTL for all traits. A number of QTL regions were associated with multiple traits, such as on linkage groups 2.2, 4.1, and 4.2 of the L93-10 linkage map and linkage groups 3.1 and 5.1 of the 7418-3 linkage map. Quantitative trait loci identified in this study may be implemented in future studies to identify markers for use in marker-assisted selection to improve heat tolerance or to help elucidate mechanisms regulating heat tolerance in creeping bentgrass.