Waterborne exposure to low concentrations of BDE-47 impedes early vascular development in zebrafish embryos/larvae
Waterborne exposure to low concentrations of BDE-47 impedes early vascular development in zebrafish embryos/larvae
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水源接触低浓度 BDE-47 会阻碍斑马鱼胚胎/幼鱼的早期血管发育
DOI：
10.1016/j.aquatox.2018.07.012
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Wei Yanhong
中科院分区：
文献类型：
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作者：
Xing Xiumei;Kang Jianmeng;Qiu Jiahuang;Zhong Xiali;Shi Xiongjie;Zhou Bingsheng;Wei Yanhong
多溴联苯醚（PBDEs）是广泛存在于各种环境基质中的持久性阻燃剂。尽管根据逐步淘汰政策，多溴二苯醚的使用量最近有所减少，但由于其持久性和长期大规模生产，在环境和生物样本中仍然发现了高含量的多溴二苯醚。发育毒性是多溴二苯醚的一个主要健康问题。然而，人们对多溴二苯醚接触对血管发育的影响仍然知之甚少。在这项研究中，我们研究了低浓度的2，2 ′，4，4 ′-四溴二苯醚（BDE-47），一个主要的多溴二苯醚同系物，在环境基质和生物区系，对早期血管发育的影响，使用斑马鱼。从受精后2小时开始，斑马鱼胚胎连续暴露于浓度为0.06、0.2、0.6 μM的水性BDE-47。使用共聚焦显微镜获得Tg（kdrl：eGFP）斑马鱼中血管的荧光图像。结果表明，在72 hpf以内的早期阶段，BDE-47暴露对孵化率、存活率、体重、体长或心率没有影响，而暴露于BDE-47的斑马鱼表现出多种血管生长受损。用BDE-47处理的胚胎中，30 hpf时完整的节间血管（ISV）百分比以剂量依赖性方式下降。BDE-47暴露导致总主静脉（CCV）的生长略有下降，但显著阻碍了CCV的重塑过程，表现为更大的CCV面积和更宽的腹侧直径。在72 hpf时，接触BDE-47会显著减少斑马鱼幼体的肠下血管（SIV）面积以及血管化卵黄面积。此外，在接触BDE-47的斑马鱼中，与血管生长和重塑相关的基因表达受到明显抑制。这些发现表明BDE-47对早期血管发育的不利影响，并证实了多溴二苯醚在体内的血管毒性。研究结果表明，斑马鱼体内正在发育的血管系统对接触BDE-47很敏感，可以作为评估早期接触多溴二苯醚的有力工具。
Polybrominated diphenyl ethers (PBDEs) are persistent flame retardants ubiquitously existing in various environment matrices. In spite of a recent reduction in use according to the phase-out policy, high levels of PBDEs are still found in both environmental and biological samples due to their persistent property and large-scale production over a long history. Developmental toxicity is a major health concern of PBDEs. However, the impact of PBDE exposure on vascular development remains poorly understood. In this study, we investigated the effect of low concentrations of 2,2′,4,4′-Tetrabromodiphenyl ether (BDE-47), a predominant PBDE congener, in environmental matrices and biota, on early vascular development using zebrafish. Zebrafish embryos were continuously exposed to waterborne BDE-47 at 0.06, 0.2, 0.6 μM starting from 2 h post-fertilization (hpf). Fluorescent images of vasculatures in Tg(kdrl:eGFP) zebrafish were acquired using a confocal microscope. The results indicated that BDE-47 exposure had no effect on hatching rate, survival, body weight, body length or heart rate in the early stage within 72 hpf, whereas zebrafish exposed to BDE-47 exhibited impairments in the growth of multiple types of blood vessels. The percentage of completed intersegmental vessels (ISV) at 30 hpf decreased in embryos treated with BDE-47 in a dose-dependent fashion. BDE-47 exposure led to a slight decrease in the growth of common cardinal vein (CCV), while dramatically hindered CCV remodeling process reflected by the larger CCV area and wider ventral diameter. BDE-47 exposure significantly reduced sub-intestinal vessels (SIV) area as well as the vascularized yolk area in zebrafish larvae at 72 hpf. In addition, the expression of genes related to vascular growth and remodeling was markedly suppressed in BDE-47-exposed zebrafish. These findings demonstrate the adverse effects of BDE-47 on early vascular development, and confirm the vascular toxicity of PBDEsin vivo. The results indicate that developing vasculature in zebrafish is sensitive to BDE-47 exposure, and may serve as a powerful tool for the assessment of early exposure to PBDEs.