RMS2 encoding a GDSL lipase mediates lipid homeostasis in anthers to determine rice male fertility
RMS2 encoding a GDSL lipase mediates lipid homeostasis in anthers to determine rice male fertility
复制标题
编码 GDSL 脂肪酶的 RMS2 介导花药中的脂质稳态以确定水稻雄性育性
DOI：
10.1104/pp.19.01487
复制
发表时间：
2020
期刊：
影响因子：
7.4
通讯作者：
Zhang Jian
中科院分区：
文献类型：
--
作者：
Zhao Juan;Long Tuan;Wang Yifeng;Tong Xiaohong;Tang Jie;Li Jinglin;Wang Huimei;Tang Liqun;Li Zhiyong;Shu Yazhou;Liu Xixi;Li Shufan;Liu Hao;Li Jialin;Wu Yongzhong;Zhang Jian
植物雄性配子发生是涉及生殖组织和孢子体组织的协调努力，其中脂质代谢起着至关重要的作用。尽管 GDSL 酯酶/脂肪酶众所周知是许多植物发育过程和胁迫反应的关键酶，但它们在生殖发育中的功能仍不清楚。在此，我们报告了水稻（Oryza sativa）中arice雄性不育2（rms2）突变体的鉴定，该突变体由于绒毡层降解、孢子体组织角质层形成以及花粉粒中外壁和中央液泡发育受损的缺陷而完全雄性不育。RMS2是基于图谱克隆的内质网定位GDSL脂肪酶基因，该基因主要在花药发育早期转录。比较代谢分析显示，在rms2中，GDSL结构域内发生了三核苷酸缺失和一个碱基取代（TTGT至A），这降低了所得蛋白质的脂质水解酶活性，并导致16种脂质成分和许多其他代谢物的含量发生显着变化。此外，在体外和体内，RMS2都是雄性育性调节因子Undevelopment Tapetum1和Perspective Tapetal Cell1的直接靶标，这表明RMS2可能是水稻雄性育性调节网络中的关键节点。这些发现揭示了GDSL在生殖发育中的功能，并为杂交水稻育种提供了有前景的基因资源。
Plant male gametogenesis is a coordinated effort involving both reproductive tissues and sporophytic tissues, in which lipid metabolism plays an essential role. Although GDSL esterases/lipases have been well known as key enzymes for many plant developmental processes and stress responses, their functions in reproductive development remain unclear. Here, we report the identification of arice male sterile2(rms2) mutant in rice (Oryza sativa), which is completely male sterile due to the defects in tapetum degradation, cuticle formation in sporophytic tissues, and impaired exine and central vacuole development in pollen grains.RMS2was map-based cloned as an endoplasmic reticulum-localized GDSL lipase gene, which is predominantly transcribed during early anther development. Inrms2, a three-nucleotide deletion and one base substitution (TTGT to A) occurred within the GDSL domain, which reduced the lipid hydrolase activity of the resulting protein and led to significant changes in the content of 16 lipid components and numerous other metabolites, as revealed by a comparative metabolic analysis. Furthermore,RMS2is directly targeted by the male fertility regulators Undeveloped Tapetum1 and Persistent Tapetal Cell1 both in vitro and in vivo, suggesting thatRMS2may serve as a key node in the rice male fertility regulatory network. These findings shed light on the function of GDSLs in reproductive development and provide a promising gene resource for hybrid rice breeding.