DERMATAN SULFATE-RICH PROTEOGLYCAN ASSOCIATES WITH RAT TAIL TENDON COLLAGEN AT THE D-BAND IN THE GAP REGION
DERMATAN SULFATE-RICH PROTEOGLYCAN ASSOCIATES WITH RAT TAIL TENDON COLLAGEN AT THE D-BAND IN THE GAP REGION
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DOI：
10.1042/bj1970213
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发表时间：
1981-01-01
影响因子：
4.1
通讯作者：
ORFORD, CR
中科院分区：
文献类型：
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作者：
SCOTT, JE;ORFORD, CR
用阳离子酞菁苷染料Cupromeronic Blue对大鼠尾腱进行染色，在临界电解质浓度法下，专门通过EM显示蛋白多糖。在蛋白酶抑制剂存在的情况下，透明质酸酶消化证实了结果。胶原蛋白用醋酸铀酰和/或磷钨酸染色，在D期显示a-e带状。蛋白多糖在胶原原纤维周围呈正交排列，其横向元素几乎全部位于间隙区的d波段。蛋白多糖可以通过胶原分子的增生或纤维的融合（通过干扰交联）来抑制纤维的径向生长，并通过占领间隙区域的孔来抑制钙化，然后用羟基磷灰石填充。
Rat tail tendon was stained with a cationic phthalocyanin dye, Cupromeronic Blue, in a critical electrolyte concentration method specifically to demonstrate proteoglycan by EM. Hyaluronidase digestion in the presence of proteinase inhibitors corroborated the results. Collagen was stained with uranyl acetate and/or phosphotungstic acid to demonstrate the banding pattern a-e in the D period. Proteoglycan was distributed about the collagen fibrils in an orthogonal array, the transverse elements of which were located almost exclusively at the d band, in the gap zone. The proteoglycan may inhibit fibril radial growth by accretion of collagen molecules or fibril fusion [through interference with cross-linking], and calcification by occupying the holes in the gap region later to be filled with hydroxylapatite.