Structure from fleeting illumination of faint spinning objects in flight
Structure from fleeting illumination of faint spinning objects in flight
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DOI：
10.1038/nphys1129
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发表时间：
2009-01-01
期刊：
影响因子：
19.6
通讯作者：
Ourmazd, Abbas
中科院分区：
文献类型：
--
作者：
Fung, Russell;Shneerson, Valentin;Ourmazd, Abbas
用强大的X射线爆发照射飞行中的粒子，以确定单分子、病毒和纳米粒子的结构，这些行动正在进行中。这将规避当前技术的重要限制，包括将分子凝聚成纯晶体的需要。然而，从未知方向的衍射“快照”重建分子结构的建议需要比下一代光源多1,000倍的信号。使用一种新的方法，我们证明了在预期的下一代X射线源强度的弱散射大分子的结构的恢复。我们的工作缩小了通过X射线方法确定单分子和纳米颗粒结构的关键差距，并开辟了在极低信号水平下重建旋转或随机取向物体结构的途径。
Moves are afoot to illuminate particles in flight with powerful X-ray bursts, to determine the structure of single molecules, viruses and nanoparticles. This would circumvent important limitations of current techniques, including the need to condense molecules into pure crystals. Proposals to reconstruct the molecular structure from diffraction 'snapshots' of unknown orientation, however, require similar to 1,000 times more signal than available from next-generation sources. Using a new approach, we demonstrate the recovery of the structure of a weakly scattering macromolecule at the anticipated next-generation X-ray source intensities. Our work closes a critical gap in determining the structure of single molecules and nanoparticles by X-ray methods, and opens the way to reconstructing the structure of spinning, or randomly oriented objects at extremely low signal levels.