Inhibitory effects of Japanese horseradish (Wasabia japonica) on the formation and genotoxicity of a potent carcinogen, acrylamide
Inhibitory effects of Japanese horseradish (Wasabia japonica) on the formation and genotoxicity of a potent carcinogen, acrylamide
复制标题
DOI：
10.1002/jsfa.8055
复制
发表时间：
2017-06-01
影响因子：
4.1
通讯作者：
Masuda, Shuichi
中科院分区：
文献类型：
--
作者：
Shimamura, Yuko;Iio, Misako;Masuda, Shuichi
背景：熟食中丙烯酰胺(AA)的形成引起了人们对人类健康的关注。AA由细胞色素P450 2E1(细胞色素P450 2E1)代谢为缩水甘草胺(GA)，形成DNA加合物。研究了山葵根、叶及其活性成分异硫氰酸烯丙酯(AIT)对AA形成和遗传毒性的抑制作用。结果：AIT可抑制AA的形成(51.8+/-4.2 mU·kg~(-1))，剂量为2 mg·L~(-1)。山葵根也抑制AA的形成(类似于减少90%)，但山葵叶在2 mg mL(-1)时不起作用。与AA处理组相比，山葵根和叶的微核细胞数分别减少约33%和24%。此外，山葵根和叶(100 mg kg(-1)体重(-1)天(-1))可减轻AA(100 mg kg-1bw(-1)天-1)引起的DNA损伤。山葵根和叶中AA诱导的细胞色素P450-2E1酶活性分别降低39%和26%。此外，山葵根和叶中催化谷胱甘肽S转移酶的活性分别提高了54%和33%。结论：山葵根和叶是抑制AA形成和遗传毒性的有效成分。(三)2016年化学工业学会
BACKGROUND: The formation of acrylamide (AA) in cooked foods has raised human health concerns. AA is metabolized by cytochrome P450 2E1 (CYP2E1) to glycidamide (GA), which forms DNA adducts. This study examined the inhibitory effects of wasabi (Japanese horseradish, Wasabia japonica) roots and leaves as well as their active component, allyl isothiocyanate (AIT), on the formation and genotoxicity of AA.RESULTS: AA formation (51.8 +/- 4.2 mu g kg(-1)) was inhibited with >= 2 mg mL(-1) of AIT. Wasabi roots also inhibited AA formation (similar to 90% reduction), but wasabi leaves were not effective at 2 mg mL(-1). Wasabi roots and leaves decreased the number of cells with micronuclei by approximately 33 and 24% respectively compared with the AA treatment group. Moreover, wasabi roots and leaves (100 mg kg(-1) body weight (BW) day(-1) for each) decreased AA (100 mg kg-1 BW day-1)-induced DNA damage. The AA-induced CYP2E1 activity was decreased by 39 and 26% with wasabi roots and leaves respectively. Further, the activity of glutathione S-transferase, which catalyzes the detoxification of AA via glutathione conjugation, increased by 54 and 33% with wasabi roots and leaves respectively.CONCLUSION: These results indicate that wasabi roots and leaves are effective ingredients for inhibiting the formation and genotoxicity of AA. (C) 2016 Society of Chemical Industry