Analysis of cholesterol levels in lipoprotein(a) with anion-exchange chromatography
Analysis of cholesterol levels in lipoprotein(a) with anion-exchange chromatography
复制标题
阴离子交换色谱法分析脂蛋白(a)中的胆固醇水平
作者：
Y. Hirowatari;H. Yoshida;Hideo Kurosawa;Y. Shimura;H. Yanai;N. Tada
我们之前建立了一种分析方法，通过阴离子交换(AEX)-HPLC法确定血清中五种主要脂蛋白类[HDL， LDL，中密度脂蛋白（IDL）， VLDL和乳糜微粒]的胆固醇水平，但脂蛋白(a) [Lp(a)]是众所周知的动脉粥样硬化疾病的危险因素，无法确定。因此，我们建立了新的AEX-HPLC分离条件，用于分析包括Lp(a)在内的6类脂蛋白的胆固醇水平。采用高效液相色谱法，以二乙胺乙基配体无孔聚合物为基柱，经高氯酸钠浓度逐步梯度洗脱，分离血清脂蛋白。在这种改进的方法中，从柱中分别洗脱HDL， LDL， IDL， VLDL，乳糜微粒和Lp(a)。通过柱后反应测定洗脱的脂蛋白的胆固醇水平。日内测定和日间测定的脂蛋白胆固醇水平变异系数分别为0.29 ~ 11.86%和0.57 ~ 11.99%。AEX-HPLC测定的Lp(a)胆固醇水平与市售免疫比浊法测定的Lp(a)蛋白量显著相关（r = 0.9503, P < 0.0001）。综上所述，该AEX-HPLC方法可有效应用于高水平Lp(a)的血清脂蛋白分析。
We previously established an analysis method for determining the cholesterol levels of five major lipoprotein classes [HDL, LDL, intermediate density lipoprotein (IDL), VLDL, and chylomicrons] in serum by an anion-exchange (AEX)-HPLC method, but lipoprotein(a) [Lp(a)], a well-known risk factor for atherosclerotic diseases, was not determinable. Therefore, we established new AEX-HPLC separation conditions for analyzing the cholesterol levels of six lipoprotein classes, including Lp(a). Serum lipoproteins were separated by HPLC with a diethylaminoethyl-ligand nonporous polymer-based column by elution with a stepwise gradient of the sodium perchlorate concentration. In this improved method, HDL, LDL, IDL, VLDL, chylomicrons, and Lp(a) were each eluted from the column. The cholesterol levels of the eluted lipoproteins were measured enzymatically by a postcolumn reaction. The within-day assay and between-day assay coefficients of variation for the lipoprotein cholesterol levels were in the ranges of 0.29–11.86% and 0.57–11.99%, respectively. The Lp(a) cholesterol levels determined by AEX-HPLC were significantly correlated with the amounts of Lp(a) protein measured by an immunoturbidimetry method available commercially (r = 0.9503, P < 0.0001). Taken together, this AEX-HPLC method may be effectively applied to the analysis of serum lipoproteins with high levels of Lp(a).
影响因子：
120.7
作者：
Danik, Jacqueline Suk;Rifai, Nader;Ridker, Paul M.
通讯作者：
Ridker, Paul M.
人载脂蛋白[a]的多态性：遗传及其分子量与脂蛋白血浆水平的关系[a]。
影响因子：
6.5
作者：
Gaubatz,JW;Ghanem,KI;GuevaraJr,J;Nava,ML;Patsch,W;Morrisett,JD
通讯作者：
Morrisett,JD
一项针对斯坦福五城项目参与者的脂蛋白（a）水平和载脂蛋白（a）大小以及冠心病风险的前瞻性病例对照研究。
DOI：
10.1161/01.atv.17.2.239
发表时间：
1997
期刊：
Arteriosclerosis, thrombosis, and vascular biology
影响因子：
--
作者：
Wild,SH;Fortmann,SP;Marcovina,SM
通讯作者：
Marcovina,SM