Synthetic analogues of marine alkaloid clathrodin differently induce phosphatidylserine exposure in monocytic cancer cells then in cancer stem cell lines
Synthetic analogues of marine alkaloid clathrodin differently induce phosphatidylserine exposure in monocytic cancer cells then in cancer stem cell lines
复制标题
海洋生物碱网格蛋白的合成类似物在单核癌细胞中与在癌症干细胞系中不同地诱导磷脂酰丝氨酸暴露
作者：
Dominik Nabergoj;Sanja Vrbek;Nace Zidar;T. Tomašič;D. Kikelj;L. P. Mašič;C. Muller
激活癌细胞的凋亡可以阻止几种癌症的发展。通过在不同的人类癌细胞系（血液、肝脏、胰腺）和癌症干细胞系（胰腺和睾丸）中进行实验，采用细胞学方法研究了从加勒比海海绵中分离的四种生物碱克罗天素合成类似物诱导细胞凋亡的动力学差异。类似物5在被测试的癌症干细胞系中具有活性，有趣的是，在单核THP-1细胞中，pan caspase抑制剂Z-VAD-fmk的存在对诱导凋亡有不同的反应。因此，在单核细胞中，与癌症干细胞相反，这种不依赖于caspase的机制可能反映了转录酶的调节，表面和细胞质之间膜的双向运输或诱导坏死。
Activation of apoptosis in cancer cells could stop the development of several cancers. Through testing in different human cancer cell lines (blood, liver, pancreas) and cancer stem cell lines (pancreas and testis), a cytomic approach was performed to investigate kinetic differences in the mechanism of proapoptotic activity induced by four synthetic analogues of clathrodin – an alkaloid isolated from Caribbean sea sponge. Active on the tested cancer stem cell lines, analogue 5 interestingly shows a differential response in apoptosis induction in the presence of pan caspase inhibitor Z-VAD-fmk in monocytic THP-1 cells. Thus in monocytic cells, contrary to cancer stem cells, this caspase independent mechanism could reflect either a scramblase modulation, a disturbed bidirectional trafficking of the membrane between the surface and cytoplasm or a necroptosis induction.
热带海绵生物碱毒素对膜钠流的影响。
DOI：
10.1016/0041-0101(94)00164-4
发表时间：
1995
期刊：
Toxicon : official journal of the International Society on Toxinology
影响因子：
--
作者：
Rentas,AL;Rosa,R;Rodríguez,AD;DeMotta,GE
通讯作者：
DeMotta,GE
影响因子：
64.8
作者：
Guenther, Claudia;Martini, Eva;Wittkopf, Nadine;Amann, Kerstin;Weigmann, Benno;Neumann, Helmut;Waldner, Maximilian J.;Hedrick, Stephen M.;Tenzer, Stefan;Neurath, Markus F.;Becker, Christoph
通讯作者：
Becker, Christoph