Dopamine D1 receptor expression is bipolar cell type-specific in the mouse retina.
Dopamine D1 receptor expression is bipolar cell type-specific in the mouse retina.
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DOI：
10.1002/cne.23932
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发表时间：
2016-07-01
期刊：
影响因子：
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通讯作者：
Ichinose T
中科院分区：
文献类型：
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作者：
Farshi P;Fyk-Kolodziej B;Krolewski DM;Walker PD;Ichinose T
在视网膜中，多巴胺是白天视力的关键分子。多巴胺由视网膜多巴胺能无突细胞释放，并通过常规突触或体积传递信号。通过体积传递，多巴胺调节视网膜神经元的所有层；然而，多巴胺如何调节双相细胞的视觉信号通路尚不清楚。在这里，我们分析了Drd1a-tdTomato BAC转基因小鼠，发现多巴胺D1受体（D1R）在视网膜双极细胞中以类型依赖的方式表达。内核层检测到强烈的tdTomato荧光，定位于1型、3b型和4型OFF双极细胞和5-2型、XBC型、6型和7型ON双极细胞。相比之下，2型、3a型、5-1型、9型和棒状双极细胞不表达Drd1a-tdTomato。其他中间神经元也表达tdTomato，包括水平细胞和一部分（25%）的AII无突细胞。不同的视觉处理途径，如颜色或运动编码的途径被认为是在视网膜双极细胞启动。我们的研究结果表明，多巴胺以一种类型依赖的方式塑造双相细胞的表现，以促进白天的视力。
In the retina, dopamine is a key molecule for daytime vision. Dopamine is released by retinal dopaminergic amacrine cells and transmits signaling either by conventional synaptic or by volume transmission. By means of volume transmission, dopamine modulates all layers of retinal neurons; however, it is not well understood how dopamine modulates visual signaling pathways in bipolar cells. Here, we analyzed Drd1a-tdTomato BAC transgenic mice and found that dopamine D1 receptor (D1R) is expressed in retinal bipolar cells in a type-dependent manner. Strong tdTomato fluorescence was detected in the inner nuclear layer and localized to type 1, 3b, and 4 OFF bipolar cells and type 5-2, XBC, 6, and 7 ON bipolar cells. In contrast, type 2, 3a, 5-1, 9, and rod bipolar cells did not express Drd1a–tdTomato. Other interneurons were also found to express tdTomato including horizontal cells and a subset (25%) of AII amacrine cells. Diverse visual processing pathways, such as color or motion-coded pathways are thought to be initiated in retinal bipolar cells. Our results indicate that dopamine sculpts bipolar cell performance in a type-dependent manner to facilitate daytime vision.