Effect of salts on the solubility of thermolysin: a remarkable increase in the solubility as well as the activity by the addition of salts without aggregation or dispersion of thermolysin.
Effect of salts on the solubility of thermolysin: a remarkable increase in the solubility as well as the activity by the addition of salts without aggregation or dispersion of thermolysin.
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盐对嗜热菌蛋白酶溶解度的影响：通过添加盐，嗜热菌蛋白酶的溶解度和活性显着增加，而不聚集或分散。
DOI：
10.1093/oxfordjournals.jbchem.a022014
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发表时间：
1998
影响因子：
2.7
通讯作者：
B. Tonomura
中科院分区：
文献类型：
--
作者：
K. Inouye;K. Kuzuya;B. Tonomura
嗜热菌蛋白酶在高浓度（1-5 M）的中性盐存在下被显著活化[Inouye，K.等（1992）J.Biochem.112，335-340]。在pH7.0和25 ℃下，用4 M NaCl使活性增强13-15倍。在这项研究中，中性盐对嗜热菌蛋白酶的溶解度的影响进行了检查。虽然在0 - 60 ℃的温度范围内，在40 mM Tris-HCl缓冲液（pH 7.5）中的溶解度仅为1.0-1.2 mg/ml，但通过加入盐，溶解度大大增加。随着NaCl浓度的增加，溶解度呈钟形行为，最大溶解度（10 mg/ml）在2.0-2.5 M NaCl。对于LiCl和NaI，随着盐浓度增加至5 M，其逐渐增加至20-50 mg/ml。在盐的存在下观察到的溶解度随着温度从0 ° C增加到60 ° C而降低，并且还随着离液阴离子效应的顺序而降低。在0-3 M NaCl的存在下，嗜热菌蛋白酶的分子量估计为33.0（+/-2.5）x 10（3），这表明在存在或不存在3 M NaCl的情况下，嗜热菌蛋白酶以单体形式存在。排除了嗜热菌蛋白酶的聚集和/或分散可能与盐的显著活化有关的可能性。
Thermolysin is remarkably activated in the presence of high concentrations (1-5 M) of neutral salts [Inouye, K. (1992) J. Biochem. 112, 335-340]. The activity is enhanced 13-15 times with 4 M NaCl at pH 7.0 and 25 degrees C. In this study, the effect of neutral salts on the solubility of thermolysin has been examined. Although the solubility was only 1.0-1.2 mg/ml in 40 mM Tris-HCl buffer, pH 7.5, in the temperature range between 0 and 60 degrees C, it was increased greatly by the addition of salts. With NaCl, the solubility showed a bell-shaped behavior with increasing NaCl concentration, and the maximum solubility (10 mg/ml) was at 2.0-2.5 M NaCl. With LiCl and NaI, it increased progressively to 20-50 mg/ml with increasing salt concentration up to 5 M. The solubility observed in the presence of salts decreased with increasing temperature from 0 to 60 degrees C, and also with the order of chaotropic anion effect. The molecular weight of thermolysin was estimated to be 33.0(+/-2.5) x 10(3) in the presence of 0-3 M NaCl, suggesting that thermolysin exists as a monomer in the presence or absence of 3 M NaCl. The possibility that aggregation and/or dispersion of thermolysin might be related to the remarkable activation by salt was ruled out.