ANTIBODY-ENHANCED DENGUE VIRUS-INFECTION IN PRIMATE LEUKOCYTES
ANTIBODY-ENHANCED DENGUE VIRUS-INFECTION IN PRIMATE LEUKOCYTES
复制标题
DOI：
10.1038/265739a0
复制
发表时间：
1977-01-01
期刊：
影响因子：
64.8
通讯作者：
OROURKE, EJ
中科院分区：
文献类型：
--
作者：
HALSTEAD, SB;OROURKE, EJ
登革病毒，1-4型，是节肢动物传播的黄病毒，在人类中引起登革休克综合征（DSS），具有感染前抗体，被动获得或源自异型感染1。虽然DSS的免疫病理机制尚未完全了解，但实验研究表明登革病毒的产生受到免疫调节。对登革热1型、3型或4型病毒具有单型免疫力的猴子，在用登革热2型病毒攻击时，其循环病毒水平显著高于类似感染的易感动物2。这可能是由于病毒在白细胞中复制所致。在受感染的猴子中，病毒经常从血沉棕黄层细胞和淋巴组织中回收3。白细胞在增强感染中的作用得到以下观察结果的进一步支持：登革热在从免疫猿或人类供体制备的外周血白细胞（PBL）培养物中容易复制，但在来自非免疫宿主的白细胞中复制较差或根本不复制4 -6。由于需要昂贵的实验宿主（猴子）或具有按时间顺序定义的登革热感染的人类供体，因此对这种现象的免疫学特异性的研究受到阻碍。在这里，我们描述了体外增强登革感染PBL的抗体。该系统为原发性登革热感染期间年幼婴儿的DSS提供了临时模型7，8。
DENGUE viruses, types 1–4, are arthropod-borne flaviviruses which cause dengue shock syndrome (DSS) in humans possessing pre-infection antibody, passively acquired or derived from heterotypic infection1. Although the immuno-pathological mechanism of DSS is not fully understood, experimental studies suggest that dengue virus production is immunologically regulated. Monkeys with monotypic immunity to dengue types 1, 3 or 4 viruses, when challenged with dengue 2, had significantly higher levels of circulating virus than did similarly infected susceptible animals2. This may be attributable to the replication of virus in leukocytes. In infected monkeys, virus was frequently recovered from buffy coat cells and from lymphatic tissues3. The role of leukocytes in enhanced infection is further supported by the observation that dengue replicates readily in cultures of peripheral blood leukocytes (PBL) prepared from immune simian or human donors, but poorly or not at all in leukocytes from non-immune hosts4–6. Studies on the immunological specificity of this phenomenon have been hindered by the requirement either for expensive experimental hosts (monkeys) or for human donors with chronologically defined dengue infections. Here we describe thein vitroenhancement of dengue infection in PBL by antibody. This system provides a provisional model for DSS in young infants during primary dengue infections7,8.