The transcriptional landscape of the yeast genome defined by RNA sequencing
The transcriptional landscape of the yeast genome defined by RNA sequencing
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DOI：
10.1126/science.1158441
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发表时间：
2008-06-06
期刊：
影响因子：
56.9
通讯作者：
Snyder, Michael
中科院分区：
文献类型：
--
作者：
Nagalakshmi, Ugrappa;Wang, Zhong;Snyder, Michael
鉴定有机体中未翻译的区域，内含子和编码区域仍然具有挑战性。我们开发了一种基于定量测序的方法，称为RNA-SEQ，用于映射转录的区域，其中互补的DNA片段经过高吞吐量测序并映射到基因组。我们应用RNA-SEQ来生成酵母基因组的高分辨率转录组图，并证明大多数（74.5％）的酵母基因组的非反抗序列被转录。我们确认了许多已知和预测的内含子，并证明了其他人没有积极使用。对于许多酵母基因，还确定了替代的起始密码子和上游开放式阅读框。我们还发现了意外的3'-最终异质性和许多重叠基因的存在。这些结果表明，酵母转录组比以前所欣赏的要复杂。
The identification of untranslated regions, introns, and coding regions within an organism remains challenging. We developed a quantitative sequencing- based method called RNA- Seq for mapping transcribed regions, in which complementary DNA fragments are subjected to high- throughput sequencing and mapped to the genome. We applied RNA- Seq to generate a high- resolution transcriptome map of the yeast genome and demonstrated that most ( 74.5%) of the nonrepetitive sequence of the yeast genome is transcribed. We confirmed many known and predicted introns and demonstrated that others are not actively used. Alternative initiation codons and upstream open reading frames also were identified for many yeast genes. We also found unexpected 3'- end heterogeneity and the presence of many overlapping genes. These results indicate that the yeast transcriptome is more complex than previously appreciated.