Circulating HOTAIR expression predicts the clinical response to neoadjuvant chemotherapy in patients with breast cancer
Circulating HOTAIR expression predicts the clinical response to neoadjuvant chemotherapy in patients with breast cancer
复制标题
循环 HOTAIR 表达预测乳腺癌患者新辅助化疗的临床反应
DOI：
10.3233/cbm-170874
复制
发表时间：
2018-01-01
影响因子：
3.1
通讯作者：
Qu, Shaohua
中科院分区：
文献类型：
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作者：
Lu, Rongzhao;Zhang, Jie;Qu, Shaohua
背景技术背景：HOTAIR长链非编码RNA在多种恶性肿瘤患者血清中均有表达，有望成为乳腺癌诊断和预后预测的新生物标志物。然而，循环HOTAIR预测对新辅助化疗（NAC）的反应的价值仍不清楚。目的：在本研究中，我们分析了治疗前循环HOTAIR水平是否预测对NAC的反应，并研究了循环HOTAIR对接受NAC治疗的乳腺癌患者无病生存期（DFS）的预后影响。采用实时荧光定量PCR检测112例乳腺癌患者NAC治疗前血清中HOTAIR水平。分析HOTAIR水平与临床病理状态及NAC疗效的相关性。结果：在112例NAC前血清标本中，高循环HOTAIR与肿瘤体积大、淋巴结阳性率高、远处转移率高相关。然而，循环HOTAIR水平与年龄、Ki 67状态或激素受体之间无显著相关性。此外，高循环HOTAIR的患者比低循环HOTAIR的患者获得更少的临床应答以及病理完全应答（p < 0.05）。Kaplan-Meier生存曲线的中位随访时间为48个月，表明高循环HOTAIR表达的患者的无病生存率低于低循环HOTAIR表达的患者（log-rank p = 0.012）。结论：高循环HOTAIR水平与乳腺癌患者对新辅助化疗的反应较低以及预后较差相关。因此，本研究为使用循环HOTAIR作为新辅助化疗反应的预测因子提供了有利的依据。
BACKGROUND: Long noncoding RNA HOTAIR has been detected in the serum of patients with various malignances and may be served as novel biomarker for diagnosis and prognosis prediction of breast cancer. However, the value of circulating HOTAIR to predict the response to neoadjuvant chemotherapy (NAC) remains unclear.OBJECTIVE: In the present study, we analyzed whether pretreatment circulating HOTAIR levels predict the response to NAC and investigated prognostic impact of circulating HOTAIR on disease-free survival (DFS) in breast cancer patients treated with NAC.METHODS: Circulating HOTAIR levels in the serum of 112 breast cancer patients before NAC were measured using quantitative real-time PCR. The correlation of circulating HOTAIR with the climcopathologic status and the response to NAC were analyzed. Kaplan-Meier survival analysis and log-rank test were used to estimate the DFS.RESULTS: In 112 serum samples obtained before NAC, high circulating HOTAIR was associated with larger tumor size, more positive lymph nodes as well as more distant metastasis. However, there was no significant correlation between the circulating HOTAIR levels and age, Ki67 status or hormone receptor. Furthermore, patients with high circulating HOTAIR achieved less clinical response as well as pathologic complete response than those with low circulating HOTAIR (p < 0.05). The Kaplan-Meier survival curve with a median follow-up of 48 months demonstrated that patients with high circulating HOTAIR expression had a worse disease-free survival than those with low circulating HOTAIR (log-rank p = 0.012).CONCLUSIONS: High circulating HOTAIR level correlates with less response to neoadjuvant chemotherapy as well as a worse prognosis in breast cancer patients. Therefore, the present study provides a favorable basis to use circulating HOTAIR as a predictor of neoadjuvant chemotherapy response.