Comparison of the receptor binding properties of contemporary swine isolates and early human pandemic H1N1 isolates (Novel 2009 H1N1)
Comparison of the receptor binding properties of contemporary swine isolates and early human pandemic H1N1 isolates (Novel 2009 H1N1)
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DOI：
10.1016/j.virol.2011.01.027
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发表时间：
2011-05-10
期刊：
影响因子：
3.7
通讯作者：
Steinhauer, David A.
中科院分区：
文献类型：
--
作者：
Bradley, Konrad C.;Jones, Cheryl A.;Steinhauer, David A.
我们利用葡聚糖微阵列技术确定了最近2009年H1N1人类大流行(PdmH1N1)早期分离株的受体结合特性，并将它们与同年北美猪流感分离株以及过去季节性H1N1人类分离株进行了比较。我们发现，pdmH1N1毒株以及所研究的猪流感分离株几乎都与α2，6连接的唾液酸多糖结合，而几乎没有检测到与α2，3连接的物种的结合。具有相同糖链骨架的化合物之间的配对比较突出了这一点，仅在其末端键的化学上不同。PdmH1N1毒株和猪分离株在受体结合谱上的总体相似性表明，HA的结合成分似乎几乎不需要适应，就可以从猪传播到人，以及随后的人到人传播。(C)2011 Elsevier Inc.保留所有权利。
We have utilized glycan microarray technology to determine the receptor binding properties of early isolates from the recent 2009 H1N1 human pandemic (pdmH1N1), and compared them to North American swine influenza isolates from the same year, as well as past seasonal H1N1 human isolates. We showed that the pdmH1N1 strains, as well as the swine influenza isolates examined, bound almost exclusively to glycans with alpha 2,6-linked sialic acid with little binding detected for alpha 2,3-linked species. This is highlighted by pair-wise comparisons between compounds with identical glycan backbones, differing only in the chemistry of their terminal linkages. The overall similarities in receptor binding profiles displayed by pdmH1N1 strains and swine isolates indicate that little or no adaptation appeared to be necessary in the binding component of HA for transmission from pig to human, and subsequent human to human spread. (C) 2011 Elsevier Inc. All rights reserved.