Preliminary X-ray crystallographic studies of methylamine dehydrogenase and methylamine dehydrogenase--amicyanin complexes from Paracoccus denitrificans.
Preliminary X-ray crystallographic studies of methylamine dehydrogenase and methylamine dehydrogenase--amicyanin complexes from Paracoccus denitrificans.
复制标题
脱氮副球菌甲胺脱氢酶和甲胺脱氢酶-氨基花青素复合物的初步 X 射线晶体学研究。
DOI：
10.1016/0022-2836(88)90134-9
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发表时间：
1988
影响因子：
5.6
通讯作者：
Husain,M
中科院分区：
文献类型：
--
作者：
Chen,L;Lim,LW;Mathews,FS;Davidson,VL;Husain,M
用气相扩散法合成了脱氮副冠藻甲胺脱氢酶单晶及甲胺脱氢酶与氨基和脱氨基-氨基的络合物。甲胺脱氢酶晶体属正交晶系P2 1 2 1 2 1，晶胞参数为a=15 1·78Å，b=135·84Å，c=5 5·14Å。这两个配合物晶体为四方晶系，空间群为P4 1 2 1 2或其对映体，晶胞参数为a=b=12 4·6Å和c=2 4 7·3Å。晶体的衍射率超过了2.8？分辨率。
Single crystals of methylamine dehydrogenase and of complexes between methylamine dehydrogenase and both amicyanin and apo-amicyanin from Paracoccus denitrificans have been prepared by the vapour diffusion method. The methylamine dehydrogenase crystals belong to the orthorhombic space group P2 1 2 1 2 1 and have unit cell parameters a= 151· 78 A ̊, b= 135· 84 A ̊ and c= 55· 14 A ̊. The two complex crystals are isomorphous and are tetragonal, space group P4 1 2 1 2 or its enantiomer, and have cell parameters a= b= 124· 6 A ̊ and c= 247· 3 A ̊. The crystals diffract to beyond 2· 8 Å resolution.