DNA barcoding in closely related species: A case study of Primula L. sect. Proliferae Pax (Primulaceae) in China
DNA barcoding in closely related species: A case study of Primula L. sect. Proliferae Pax (Primulaceae) in China
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密切相关物种的 DNA 条形码：报春花组的案例研究。
DOI：
10.1111/j.1759-6831.2011.00115.x
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发表时间：
2011-05-01
影响因子：
3.7
通讯作者：
Ge, Xue-Jun
中科院分区：
文献类型：
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作者：
Yan, Hai-Fei;Hao, Gang;Ge, Xue-Jun
DNA条形码是一种通过分析一个或几个短的标准化DNA序列来识别物种的方法。植物条形码技术面临着特殊的挑战，尤其是在区分近亲物种方面。因此，迫切需要评估候选基因座的物种区分性能，特别是近缘物种，以补充rbcL + matK组合作为陆地植物核心条形码的建议。本研究以报春花（Primula sect. Proliferae Pax） 12种48个个体为样本，对8个主要候选条形码位点（matK、rbcL、rpoB、rpoC1、trnH-psbA、psbK-psbI、attf - atph和内部转录间隔物（ITS））的性能进行了评价。核心组合rbcL + matK在增殖科中只有50%的物种分辨力。ITS、trnH-psbA和psbK-psbI作为单位点条形码，在种内和种间差异、单一性程度和序列相似性方面表现良好。与rbcL + matK单独或联合使用时，内转录间隔子均表现出最高的遗传分化和最佳的区分力（83.3%）。我们建议评估ITS在其他物种条形码中的应用。从长远来看，低拷贝或单拷贝核区域将提供更复杂的条形码工具，尽管需要进一步的研究来找到合适的位点。
DNA barcoding is a method of identifying species by analyzing one or a few short standardized DNA sequences. There are particular challenges in barcoding plants, especially for distinguishing closely related species. Hence, there is an urgent need to evaluate the performance of candidate loci for distinguishing between species, especially closely related species, to complement the rbcL + matK combination suggested as the core barcode for land plants. We sampled 48 individuals representing 12 species in Primula sect. Proliferae Pax in China to evaluate the performance of eight leading candidate barcode loci (matK, rbcL, rpoB, rpoC1, trnH-psbA, psbK-psbI, atpF-atpH, and internal transcribed spacer (ITS)). The core combination rbcL + matK gave only 50% species resolution in sect. Proliferae. In terms of intraspecies and interspecies divergence, degree of monophyly, and sequence similarity, ITS, trnH-psbA, and psbK-psbI showed good performance as single-locus barcodes. Internal transcribed spacer displayed the highest genetic divergence and best discriminatory power, both alone and in combination with rbcL + matK (83.3% species resolution). We recommend evaluating the use of ITS for barcoding in other species. Low or single copy nuclear regions would provide more sophisticated barcoding tools in the long term, even though further research is required to find suitable loci.