Assembly and Assessment of DNA Scaffolded Vaccines.
Assembly and Assessment of DNA Scaffolded Vaccines.
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DNA 支架疫苗的组装和评估。
DOI：
10.1007/978-1-4939-3389-1_21
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Chang,Yung
中科院分区：
文献类型：
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作者：
Liu,Xiaowei;Wang,Lili;Yan,Hao;Chang,Yung
疫苗在预防许多危及生命的传染病方面发挥着重要作用。为了满足疫苗接种治疗多种疾病的需求，合理的疫苗设计已被认为是开发安全有效疫苗的理想和必要策略。DNA纳米结构在设计和构建合成疫苗方面具有优势，因为它们具有强大的自组装能力、可编程性和对复杂组织的精确控制，以及它们固有的佐剂活性。在这里，我们描述了DNA支架疫苗复合物的模块化组装，由模型抗原、链亲和素和佐剂、CpG寡核苷酸组成。研究发现，dna组装疫苗可引起强烈的抗原特异性抗体反应，但几乎没有不良反应。可以想象，该疫苗平台可以进一步优化以提高免疫原性，并扩展到各种亚单位疫苗的构建中。
Vaccines play an important role in preventing many life-threatening infectious diseases. To meet the demand of vaccination for treating a wide range of diseases, rational vaccine design has been recognized as a desirable and necessary strategy for development of safe and effective vaccines. DNA nanostructures are advantageous in the design and construction of synthetic vaccines, owing to their robust self-assembly, programmability, and precision control in complex organization, as well as their intrinsic adjuvant activity. Here, we describe a modular assembly of DNA scaffolded vaccine complex, composing of a model antigen, streptavidin, and adjuvant, CpG oligonucleotide. The DNA-assembled vaccines were found to elicit strong antigen-specific antibody responses, but causing little or no adverse reactions. Conceivably, this vaccine platform can be further optimized for improved immunogenicity and extended to the construction of various subunit vaccines.