The abundance of Akkermansia muciniphila and its relationship with sulphated colonic mucins in health and ulcerative colitis
The abundance of Akkermansia muciniphila and its relationship with sulphated colonic mucins in health and ulcerative colitis
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DOI：
10.1038/s41598-019-51878-3
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发表时间：
2019-10-30
影响因子：
4.6
通讯作者：
O'Connell, P. Ronan
中科院分区：
文献类型：
--
作者：
Earley, Helen;Lennon, Grainne;O'Connell, P. Ronan
嗜粘蛋白阿克曼氏菌利用结肠粘蛋白作为其底物。溃疡性结肠炎（UC）中的硫酸化粘蛋白减少，粘液凝胶层（MGL）中硫酸化粘蛋白的相对比例也减少。目前尚不清楚这些现象是否相关，但由于缺乏底物，硫酸化粘蛋白减少可能导致丰度降低。本研究的目的是量化A。MGL内的嗜粘蛋白菌，并将这些发现与炎症标志物和存在的硫粘蛋白的相对比例相关联。从20名活动期UC（AC）患者、14名静止期UC（QUC）患者和20名健康对照（HC）患者中获得结肠活检和粘液刷检。A.通过RT-PCR测定嗜粘蛋白丰度。高铁二胺碱性蓝染色用于组织学分析。AC患者的A丰度降低。与HC和QUC相比，A.嗜粘蛋白丰度和硫酸化粘蛋白的较高百分比（rho 0.546，p = 0.000）。低丰度的A.嗜粘蛋白血症与较高的炎症评分相关（rho = 0.294（p = 0.001））。本研究证实了A.嗜粘蛋白与炎症的关系以及A. MGL中的硫酸化粘蛋白的丰度和百分比。
Akkermansia muciniphila utilises colonic mucin as its substrate. Abundance is reduced in ulcerative colitis (UC), as is the relative proportion of sulphated mucin in the mucus gel layer (MGL). It is unknown if these phenomena are related, however reduced sulphated mucins could contribute to reduced abundance, owing to a lack of substrate. The aim of this study was to quantify A. muciniphila within the MGL and to relate these findings with markers of inflammation and the relative proportion of sulphomucin present. Colonic biopsies and mucus brushings were obtained from 20 patients with active UC (AC), 14 with quiescent UC (QUC) and 20 healthy controls (HC). A. muciniphila abundance was determined by RT-PCR. High iron diamine alcian-blue staining was performed for histological analysis. Patients with AC had reduced abundance of A. muciniphila compared to HC and QUC. A positive association was found between A. muciniphila abundance and higher percentage of sulphated mucin (rho 0.546, p = 0.000). Lower abundances of A. muciniphila correlated with higher inflammatory scores (rho = 0.294 (p = 0.001)). This study confirms an inverse relationship between A. muciniphila and inflammation and a positive association between A. muciniphila abundance and percentage of sulfated mucin in the MGL.