Mantle-to-shell CaCO3 transfer during shell repair at different hydrostatic pressures in the deep-sea vent mussel Bathymodiolus azoricus (Bivalvia: Mytilidae)
Mantle-to-shell CaCO3 transfer during shell repair at different hydrostatic pressures in the deep-sea vent mussel Bathymodiolus azoricus (Bivalvia: Mytilidae)
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DOI：
10.1007/s00227-009-1140-2
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发表时间：
2009-04-01
期刊：
影响因子：
2.4
通讯作者：
Azevedo, Carlos
中科院分区：
文献类型：
--
作者：
Kadar, Eniko;Lobo-da-Cunha, Alexandre;Azevedo, Carlos
本文对2007年5月在大西洋中脊Menez Gwen热液喷口（31A°31′w, 37A°50′n） 850 m深处采集的热液贻贝（Bathymodiolus azoricus）进行了碳酸钙转移的实验研究。在四个为期10天的实验中，每组贻贝被维持在大气压力下或重新加压到与其发生地点相关的深度，即Menez Gwen的850米深度，Lucky Strike的1750米深度（西经31度31分，北纬37度18分）和Rainbow的2300米深度（西经31度31分，北纬36度13分）。实验贻贝在损伤后第7天和第10天穿孔，固定套膜组织进行光和透射电镜研究。同时，对诱导部位的头外液（EPF）中的血细胞进行了研究。在第7天，响应在地幔边缘中部褶皱最为强烈，可能与静水压力成正比。在第10天，面向体腔的被膜表面上皮细胞产生大量的有机分泌物，这些分泌物贪婪地结合钙。在地幔组织内可见到血细胞的迁移，并且在地幔表面面向壳的血细胞具有钙阳性的颗粒含量。在损伤部位的EPF中检测到大血细胞，其中一些显示免疫反应的证据，而另一些显示钙阳性颗粒含量。这些结果表明，在这些深海贻贝中，血细胞参与了壳的修复，就像在一些淡水和浅海软体动物中一样。
Calcium carbonate transfer was experimentally examined in hydrothermal mussels Bathymodiolus azoricus, which were collected from 850 m depth at Menez Gwen hydrothermal vent site (31A degrees 31'W, 37A degrees 50'N) on the Mid Atlantic Ridge in May 2007. In each of four 10-day experiments, groups of mussels were maintained at atmospheric pressure or re-pressurised to depths relevant to their site of occurrence, i.e. 850 m depth at Menez Gwen, 1,750 m at Lucky Strike (31A degrees 31'W, 37A degrees 18'N) and 2,300 m at Rainbow (31A degrees 31'W, 36A degrees 13'N). The shells of experimental mussels were perforated and mantle tissue was fixed for light and TEM studies at days 7 and 10 following the injury. Simultaneously, haemocytes from the extrapallial fluid (EPF) at the site of induction were studied. At day 7 the response was most intense in the middle fold of the mantle margin and possibly proportional to hydrostatic pressure. At day 10 the epithelial cells on the mantle surface facing the body cavity produced copious organic secretions that avidly bound calcium. Haemocyte migration was noted within the mantle tissue, and the haemocytes at the mantle surface facing the shell had a Ca-positive granular content. Large haemocytes were detected in the EPF at the injury site, and some showed evidence of an immune reaction while others showed Ca-positive granular content. These results suggest that haemocytes are involved in shell repair in these deep-sea mussels just as in some freshwater and shallow marine molluscs.