Differential protein expression on the cell surface of colorectal cancer cells associated to tumor metastasis
Differential protein expression on the cell surface of colorectal cancer cells associated to tumor metastasis
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DOI：
10.1002/pmic.200900441
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发表时间：
2010-03-01
期刊：
影响因子：
3.4
通讯作者：
Ignacio Casal, J.
中科院分区：
文献类型：
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作者：
Luis Luque-Garcia, Jose;Luis Martinez-Torrecuadrada, Jorge;Ignacio Casal, J.
进展至转移是结直肠癌（CRC）生存的关键点。然而，与CRC转移相关的蛋白质组目前知之甚少。在这项研究中，我们在细胞培养物中使用氨基酸稳定同位素标记来比较两种CRC细胞系：KM 12 C和KM 12 SM，代表低转移性和高转移性，以发现和量化蛋白表达的差异，主要是在细胞表面水平。在生物素化之后，接着亲和纯化，通过SDS-PAGE分离膜蛋白，并使用纳流LC-ESI-LTQ分析。总共鉴定了291种膜和膜相关蛋白（p值）
Progression to metastasis is the critical point in colorectal cancer (CRC) survival. However, the proteome associated to CRC metastasis is very poorly understood at the moment. In this study, we used stable isotope labeling by amino acids in cell culture to compare two CRC cell lines: KM12C and KM12SM, representing poorly versus highly metastatic potential, to find and quantify the differences in protein expression, mostly at the cell surface level. After biotinylation followed by affinity purification, membrane proteins were separated by SDS-PAGE and analyzed using nanoflow LC-ESI-LTQ. A total of 291 membrane and membrane-associated proteins were identified with a p value