Transient increase in the transcript levels of γ-tubulin complex genes during reorientation of cortical microtubules by gravity in azuki bean (Vigna angularis) epicotyls
Transient increase in the transcript levels of γ-tubulin complex genes during reorientation of cortical microtubules by gravity in azuki bean (Vigna angularis) epicotyls
复制标题
DOI：
10.1007/s10265-008-0179-3
复制
发表时间：
2008-09-01
影响因子：
2.8
通讯作者：
Hoson, Takayuki
中科院分区：
文献类型：
--
作者：
Soga, Kouichi;Kotake, Toshihisa;Hoson, Takayuki
在超重力作用下，红豆上胚轴横向微管细胞的比例降低，纵向微管细胞的比例增加。γ -微管蛋白复合物编码基因VaTUG和VaGCP3的表达随着重力条件的变化而瞬间升高。镧和钆离子是机械敏感钙离子渗透通道（机械受体）的潜在阻滞剂，可以抑制微管的重新定向，并通过超重力上调VaTUG和VaGCP3的表达。这些结果表明，机械感受器可能感知重力信号，从而导致γ -微管复合物基因转录水平的短暂增加和皮质微管的重新定向。
By hypergravity treatment, the percentage of cells with transverse microtubules was decreased, while that with longitudinal microtubules was increased in azuki bean (Vigna angularis) epicotyls. The expression of genes encoding gamma-tubulin complex (VaTUG and VaGCP3) was increased transiently in response to changes in the gravitational conditions. Lanthanum and gadolinium ions, potential blockers of mechanosensitive calcium ion-permeable channels (mechanoreceptors), nullified reorientation of microtubules as well as up-regulation of expression of VaTUG and VaGCP3 by hypergravity. These results suggest that mechanoreceptors may perceive the gravity signal, which leads to a transient increase in the transcript levels of gamma-tubulin complex genes and reorientation of cortical microtubules.