Synthesis and biological actions of melanin concentrating hormone.
Synthesis and biological actions of melanin concentrating hormone.
复制标题
黑色素浓缩激素的合成及其生物学作用。
DOI：
10.1016/s0006-291x(84)80077-7
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发表时间：
1984
影响因子：
3.1
通讯作者：
Hadley,ME
中科院分区：
文献类型：
--
作者：
Wilkes,BC;Hruby,VJ;Sherbrooke,WC;Castrucci,AM;Hadley,ME
合成了一种黑色素（黑素体）浓缩激素MCH，并详细介绍了其合成方法。该七肽H-Asp-Thr-Met-Arg-Cys-Met-Val-Gly-Arg-Val-Tyr-Arg-Pro-Cys-Trp-Glu-Val-OH可刺激所研究的所有硬骨鱼种类的黑色素细胞内的黑色素小体浓度（向心聚集）。黑素体对MCH的聚集反应不像对去甲肾上腺素（NE）的反应那样被Dibenamine阻断，这表明黑素体对MCH和NE的聚集反应是通过不同的受体介导的。等摩尔浓度的α-促黑素细胞激素（α-MSH）可逆转黑素小体对MCH的聚集反应。相比之下，MCH刺激了青蛙（Ranapipiens）和蜥蜴（Anolis carolinensis）黑色素细胞内的黑素体分散（离心运动）。因此，根据所研究的物种，MCH表现出黑色素体的集中和分散作用。
A melanin (melanosome) concentrating hormone, MCH, was synthesized and the methodologyfor its synthesis is detailed.This heptadecapeptide, H-Asp-Thr-Met-Arg-Cys-Met-Val-Gly-Arg-Val-Tyr-Arg-Pro-Cys-Trp-Glu-Val-OH, stimulated melanosome concentration (centripetal aggregation) within melanophores of all species of teleost fishes studied. Melanosome aggregation in response to MCH was not blocked by Dibenamine as was the response to norepinephrine (NE), demonstrating that melanosome aggregating responses to MCH and NE are mediated through separate receptors. Melanosome aggregation in response to MCH was reversed by an equimolar concentration of α-melanocyte stimulating hormone (α-MSH). In contrast, MCH stimulated melanosome dispersion (centrifugal movement) within melanophores of a frog (Ranapipiens) and a lizard (Anolis carolinensis). Therefore, MCH exhibits both melanosome concentrating and dispersing actions depending upon the species studied.