LEVELS OF CIRCULATING COLLAGENASE, STROMELYSIN-1, AND TISSUE INHIBITOR OF MATRIX METALLOPROTEINASES-1 INPATIENTS WITH RHEUMATOID-ARTHRITIS - RELATIONSHIP TO SERUM LEVELS OF ANTIGENIC KERATAN SULFATE AND SYSTEMIC PARAMETERS OF INFLAMMATION
LEVELS OF CIRCULATING COLLAGENASE, STROMELYSIN-1, AND TISSUE INHIBITOR OF MATRIX METALLOPROTEINASES-1 INPATIENTS WITH RHEUMATOID-ARTHRITIS - RELATIONSHIP TO SERUM LEVELS OF ANTIGENIC KERATAN SULFATE AND SYSTEMIC PARAMETERS OF INFLAMMATION
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DOI：
10.1002/art.1780380803
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发表时间：
1995-08-01
影响因子：
--
通讯作者：
THONAR, EJMA
中科院分区：
文献类型：
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作者：
MANICOURT, DH;FUJIMOTO, N;THONAR, EJMA
Objective.检测类风湿关节炎（RA）患者和年龄匹配的对照组血清基质金属蛋白酶-1（MMP-1）、基质金属蛋白酶-3（MMP-3）和基质金属蛋白酶组织抑制剂-1（TIMP-1）水平，并确定这些水平与血清硫酸角质素抗原（KS）水平以及疾病活动的其他生化和临床指标的相关性。采用免疫组化法检测MMP-1、MMP-3、TIMP-1和KS抗原水平，根据Steinbrocker标准进行关节功能评分，并检测红细胞沉降率（ESR）和C反应蛋白（GRIP）水平。在RA患者中，MMP-3和TIMP-1水平显著升高，并与ESR和CRP水平密切相关，但与放射学或功能性关节评分无关。RA患者血清KS抗原水平显著降低，与全身炎症指标及血清TIMP-1水平呈负相关。结论：RA患者血清MMP-3、TIMP-1水平升高，可能反映了全身炎症反应。TIMP-1和抗原KS的血清水平之间的负相关性表明，TIMP-1合成的上调可能是负责在严重的炎症变化的患者中的软骨聚集蛋白聚糖catalysts的明显抑制。
Objective. To measure serum levels of matrix metalloproteinase-1 (MMP-1), matrix metalloproteinase- 3 (MMP-3), and tissue inhibitor of MMP-1 (TIMP-1) in patients with rheumatoid arthritis (RA) and in age-matched control subjects, and to determine how these correlate with serum levels of antigenic keratan sulfate (KS) and other biochemical and clinical indicators of disease activity.Methods. Immunoassays were used to measure levels of MMP-1, MMP-3, TIMP-1, and antigenic KS, Radiologic and functional joint scores were based upon Steinbrocker's criteria, Erythrocyte sedimentation rates (ESR) and levels of C-reactive proteins (GRIP) were measured.Results. In RA patients, levels of MMP-3 and TIMP-1 were significantly increased, and strongly correlated with the ESR and CRP levels but,not with radiologic or functional joint scores. Levels of antigenic KS were significantly lower in RA patients and correlated negatively with systemic parameters of inflammation and serum levels of TIMP-1.Conclusions, The increase in serum levels of MMP-3 and TIMP-1 appears to reflect systemic inflammation in RA. The inverse correlation between serum levels of TIMP-1 and antigenic KS suggests that an upregulation of TIMP-1 synthesis might be responsible for the apparent suppression of cartilage aggrecan catabolism in patients with severe inflammatory changes.