ACETYL TRANSPORT MECHANISMS - METABOLISM OF N-ACETYL-L-ASPARTIC ACID IN NON-NERVOUS TISSUES OF RAT
ACETYL TRANSPORT MECHANISMS - METABOLISM OF N-ACETYL-L-ASPARTIC ACID IN NON-NERVOUS TISSUES OF RAT
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DOI：
10.1016/0005-2760(68)90101-x
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发表时间：
1968-01-01
期刊：
影响因子：
--
通讯作者：
DADAMO, AF
中科院分区：
文献类型：
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作者：
BENUCK, M;DADAMO, AF
本文研究了N-[1- 14 c]-L-天冬氨酸在离体大鼠肝、肾、心脏和乳腺组织中的利用和转运。脂肪酸标记与CO2标记的比率与所检查的组织中的乙酸盐相同。在这些组织中观察到利用模式的显著差异。在肾脏切片中，超过85%的添加的放射性乙酰氨基酸被转化为14 CO2，而在乳腺实验中，大部分标记出现在脂肪酸中。肝脏和心脏的模式与肾脏中观察到的模式相似。转运实验表明，所检查的组织的摄取变化很大;最值得注意的是肾脏，其中超过90%的添加乙酰氨基酸在孵育10分钟内被吸收。这些结果表明，N-乙酰天冬氨酸水解酶存在于心脏，肝脏和乳腺除了以前发现的酰基转移酶在肾脏和大脑。用标记葡萄糖、乙酸和天冬氨酸研究了N-乙酰-L-天冬氨酸在乳腺和肾脏中的生物合成。这些研究表明，尽管这些组织中存在该化合物的活跃代谢，但未合成该乙酰基氨基酸。这表明，如果这些组织对N-乙酰基-天冬氨酸有任何需求，它是由大脑提供的。
The utilization and transport of N-[1-14c]-L-aspartic acid by liver, kidney, heart and mammary gland tissues of the rat was studied in vitro. The ratio of fatty acid labeling to that of CO2 paralleled that found with acetate for the tissues examined. Significant differences in the pattern of utilization were observed in those tissues. With kidney slices, over 85% of the added radioactive acetyl amino acid was converted to 14CO2 while in the mammary gland experiment most of the labeling appeared in the fatty acid. The pattern for liver and heart was similar to that observed in the kidney. Transport experiments showed a wide variation in uptake by the tissues examined; most notable is the kidney, where over 90% of the added acetyl amino acid is taken up within 10 min. of incubation. These results show that N-acetyl-aspartic acid hydrolases exist in the heart, liver and mammary gland in addition to the acylases previously found in the kidney and brain. The biosynthesis of N-acetyl-L-aspartic acid in mammary gland and kidney was investigated with labeled glucose, acetate and aspartic acid. These studies indicate that, although an active metabolism for the compound exists in these tissues, no synthesis of this acetyl amino acid occurs. This suggests that if these tissues have any requirement for N-acetyl-aspartic acid it is supplied by the brain.