How to Measure Export via Bacterial Multidrug Resistance Efflux Pumps.
How to Measure Export via Bacterial Multidrug Resistance Efflux Pumps.
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DOI：
10.1128/mbio.00840-16
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发表时间：
2016-07-05
期刊：
影响因子：
6.4
通讯作者：
Piddock LJ
中科院分区：
文献类型：
--
作者：
Blair JM;Piddock LJ
细菌多药耐药（MDR）外排泵是抗生素耐药的重要机制，是许多病原体引起感染所必需的。它们还被用于改进微生物生物技术过程，包括生物燃料生产。因此，许多专业的科学家必须能够准确地测量外排活动。然而，已经描述了无数的方法，最合适的方法并不总是明确的。在科学文献中，许多方法被误用或产生的数据被误解。测量外排活性的方法可分为两组，(i)直接测量外排的方法和（ii）测量底物在细胞内积累的方法，然后用底物来推断外排活性。在这里，我们回顾了测量外排的方法，并探讨了该领域的最新进展，包括单细胞或无细胞技术和质谱，这些技术被用来提供有关外排泵活性的更详细信息。
Bacterial multidrug resistance (MDR) efflux pumps are an important mechanism of antibiotic resistance and are required for many pathogens to cause infection. They are also being harnessed to improve microbial biotechnological processes, including biofuel production. Therefore, scientists of many specialties must be able to accurately measure efflux activity. However, myriad methodologies have been described and the most appropriate method is not always clear. Within the scientific literature, many methods are misused or data arising are misinterpreted. The methods for measuring efflux activity can be split into two groups, (i) those that directly measure efflux and (ii) those that measure the intracellular accumulation of a substrate, which is then used to infer efflux activity. Here, we review the methods for measuring efflux and explore the most recent advances in this field, including single-cell or cell-free technologies and mass spectrometry, that are being used to provide more detailed information about efflux pump activity.