Ceratocystis canker resistance in BC1 populations of interspecific hybridization of fig (Ficus carica) and F. erecta
Ceratocystis canker resistance in BC1 populations of interspecific hybridization of fig (Ficus carica) and F. erecta
复制标题
无花果 (Ficus carica) 和 F.ectera 种间杂交的 BC1 种群对角囊藻 (Ceratocystis) 溃疡病的抗性
DOI：
10.1016/j.scienta.2019.03.039
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发表时间：
2019
影响因子：
4.3
通讯作者：
Jikumaru Shota
中科院分区：
文献类型：
--
作者：
Yakushiji Hiroshi;Morita Takeshige;Jikumaru Shota
长喙壳溃疡病是无花果的一种严重病害。主要由土传真菌Ceratocystis ficicola引起。该病害主要侵入无花果的根部和树干，最终导致无花果死亡。卡里卡×F.（F.erecta）对该菌具有极强的抗性。由于与F.卡里卡湾F. carica。F1杂种由于根系紊乱而不能用作砧木。然后，通过人工授粉，以四种常见无花果为种子亲本，与F1型无花果为花粉亲本进行回交（BC1）。BC1代叶片形态与F.比F.直立人F1。BC1代的硬枝和嫩枝扦插苗容易生根。BC_1群体接种C. ficicicolaby直接伤害的拍摄。BC1群体的抗病性和感病性分离符合1：1孟德尔分离模型。用4个抗病的BC_1代单株的插条进行伤口接种试验，接种后100天，所有供试株系均健康不枯萎。这一结果表明，直立镰刀菌对长喙壳溃疡病的抗性受一对显性基因控制。BC1后代与无花果嫁接亲和性较好，表明抗溃疡病BC1是一种实用的无花果砧木。
Ceratocystis canker is a serious disease in fig (Ficus caricaL.) caused primarily by the soil borne fungusCeratocystis ficicola. The disease mainly invades the roots and trunks of figs, eventually killing the tree.Ficus erectaand F1(F. carica×F. erecta) were found to be extremely resistant to this fungus.F. erectais not practical as a rootstock due to extremely low grafting compatibility withF. caricabetweenF. carica. F1hybrids had been found to be unusable as a rootstock because of a root disorder. Then, we performed backcross hybridizations (BC1) between four common figs (seed parent) and F1caprifig type (pollen parent) through artificial pollination. The leaf morphology of BC1progeny was similar to that ofF. caricarather than those ofF. erectaor F1. Hardwood and softwood cuttings of BC1progeny readily rooted. BC1populations were inoculated withC. ficicolaby direct wounding of the shoot. The segregation ratios of resistance and susceptibility in BC1populations were consistent with a 1:1 Mendelian model. In a wound inoculation experiment using cuttings from 4 BC1individuals identified as being disease resistant, all examined lines were healthy and unwithered 100 days after inoculation. This result suggests that Ceratocystis canker resistance inF. erectais controlled by a single dominant gene. BC1progeny exhibited sufficient graft compatibility with fig, indicating that Ceratocystis canker-resistant BC1would be a practical fig rootstock source.