Effects of methionine sulfoximine on the enzymes of glutamate metabolism in isolated astrocytes of rat brain.
Effects of methionine sulfoximine on the enzymes of glutamate metabolism in isolated astrocytes of rat brain.
复制标题
蛋氨酸亚砜亚胺对大鼠脑离体星形胶质细胞谷氨酸代谢酶的影响。
DOI：
10.1016/0006-2952(83)90137-5
复制
发表时间：
1983
影响因子：
5.8
通讯作者：
C. Murthy
中科院分区：
文献类型：
--
作者：
G. Subbalakshmi;C. Murthy
从正常大鼠和注射了能抑制谷氨酰胺合成酶和诱导阿尔茨海默病II型星形胶质细胞的强效惊厥剂蛋氨酸亚硫胺(MSO)的大鼠脑内分离的星形胶质细胞中，对谷氨酸代谢酶进行了测定。加入MSO后，小鼠的湿重、干重、DNA、RNA、蛋白质含量以及谷氨酸脱氢酶和天冬氨酸氨基转移酶的活性均升高。MSO的代谢作用与氨不同，谷氨酸脱氢酶活性下降，谷氨酰胺合成酶活性升高。根据这些结果，推测这两种酶的功能可能存在相反的关系。这种关系将有助于防止氨解毒过程中特定细胞隔间中能量池的耗尽。
The enzymes of glutamate metabolism were estimated in astrocytes isolated from brains of normal rats and those injected with the potent convulsant, methionine sulfoximine (MSO), which inhibits glutamine synthetase and induces Alzheimer type II astrocytosis. The wet weight, dry weight; contents of DNA, RNA, protein and the activities of glutamate dehydrogenase and aspartate aminotransferase were elevated following MSO administration. The metabolic effects of MSO were found to be different from those of ammonia wherein a fall in the activity of glutamate dehydrogenase and an increase in the activity of glutamine synthetase was noticed. Based on these results it is suggested that there might be an inverse relationship in the functioning of these two enzymes. Such a relationship would help in preventing the depletion of energy pools in a given cellular compartment during ammonia detoxification.