Multi-channel fiber photometry for population neuronal activity recording
Multi-channel fiber photometry for population neuronal activity recording
复制标题
用于群体神经元活动记录的多通道光纤光度测定
DOI：
10.1364/boe.6.003919
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发表时间：
2015-10-01
影响因子：
3.4
通讯作者：
Fu, Ling
中科院分区：
文献类型：
--
作者：
Guo, Qingchun;Zhou, Jingfeng;Fu, Ling
纤维光度学作为一种记录行为动物遗传上确定的神经元群体的方便工具，在神经科学家中越来越受欢迎。在这里，我们报告的多通道光纤测光系统的发展，同时监测神经活动在几个脑区的动物或在不同的动物。在该系统中，电流镜调制激发光并将其循环耦合到各个多模光纤束。单个光电探测器收集激发光，光纤束组件和扫描器的配置决定了总通道数。我们证明，该系统表现出可忽略不计的通道之间的串扰和光学信号可以同时采样，每个通道的采样率至少为100 Hz，这是足够的记录钙信号。使用该系统，我们成功地记录了GCaMP6荧光信号从双侧桶皮质的头部限制在双通道模式，和眶额皮质的多个自由移动的小鼠在三通道模式。多通道光纤测光系统将是一个有价值的工具，在给定的动物和不同的相互作用的个人的不同脑区的群体活动的同时记录。(C)2015美国光学学会
Fiber photometry has become increasingly popular among neuroscientists as a convenient tool for the recording of genetically defined neuronal population in behaving animals. Here, we report the development of the multi-channel fiber photometry system to simultaneously monitor neural activities in several brain areas of an animal or in different animals. In this system, a galvano-mirror modulates and cyclically couples the excitation light to individual multimode optical fiber bundles. A single photodetector collects excited light and the configuration of fiber bundle assembly and the scanner determines the total channel number. We demonstrated that the system exhibited negligible crosstalk between channels and optical signals could be sampled simultaneously with a sample rate of at least 100 Hz for each channel, which is sufficient for recording calcium signals. Using this system, we successfully recorded GCaMP6 fluorescent signals from the bilateral barrel cortices of a head-restrained mouse in a dual-channel mode, and the orbitofrontal cortices of multiple freely moving mice in a triple-channel mode. The multi-channel fiber photometry system would be a valuable tool for simultaneous recordings of population activities in different brain areas of a given animal and different interacting individuals. (C) 2015 Optical Society of America