CATARACTS IN AGING RAT LENS - MORPHOLOGY AND ACID-PHOSPHATASE HISTOCHEMISTRY OF INCIPIENT FORMS
CATARACTS IN AGING RAT LENS - MORPHOLOGY AND ACID-PHOSPHATASE HISTOCHEMISTRY OF INCIPIENT FORMS
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DOI：
10.1016/0014-4835(78)90165-3
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发表时间：
1978-01-01
影响因子：
3.4
通讯作者：
GORTHY, WC
中科院分区：
文献类型：
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作者：
GORTHY, WC
形态学和某些组织化学的变化，之前和伴随白内障与老化的大鼠进行了研究。白内障分为两种类型：核上型（SNC）和后囊下型（PSC）。分别与前透镜缝线系统相关的初始生物显微镜病变归因于浅表纤维细胞末端的细胞学改变。两种类型的白内障共同的皮质改变包括节段性细胞肿胀和各种球状和多层包涵体的形成。与SNC，大的多层体对应于前缝空泡混浊，而肿胀和dissocation的纤维细胞体与层状分离，病变最终出现在外周皮质的形成。后囊下细胞间空泡，其中许多开发了一个层状的外部结构，是PSC的早期形式的特征。一种酸性磷酸酶，作为溶酶体水解酶的标志物，在早期形式的变性晶状体的细胞间隙中被证明。细胞外酶的主要来源是上皮和皮质内的高尔基体/溶酶体系统，但不排除皮质滑面内质网。白内障初期皮质纤维的肿胀和变性是由位于细胞间的水解酶引起的。老年大鼠透镜作为人类老年性白内障模型的价值被鼠类和人类疾病形式之间的许多相似性所加强。
Morphological and certain histochemical changes that precede and accompany cataracts associated with aging in the rat were studied. The cataracts were of 2 types: supranuclear (SNC) and posterior subcapsular (PSC). The initial biomicroscopic lesions, which were associated with the anterior lens suture systems, respectively, were attributed to cytological alterations of the ends of the superficial fiber cells. Cortical alterations common to both types of cataract included segmental cellular swelling and the formation of assorted globular and multilamellar inclusions. With SNC, large multilamellar bodies corresponded to anterior sutural vacuolar opacities; while swelling and dissocation of the fiber cell bodies were associated with the formation of lamellar separations, lesions which eventually appeared in the peripheral cortex. Posterior subcapsular intercellular vacuoles, many of which developed a lamellated outer structure, were characteristic of the early form of PSC. An acid phosphatase which served as a marker for lysosomal hydrolases was demonstrated in the intercellular spaces of lenses with early forms of degeneration. The major source of the extracellular enzyme was the Golgi/lysosomal system within the epithelium and cortex, although the cortical smooth endoplasmic reticulum was not ruled out. The swelling and degeneration of cortical fibers occurring in the initial stages of the cataracts were caused by intercellularly located hydrolases. The value of the aged rat lens as a model for human senile cataract was reinforced by the many similarities between the murine and human forms of the diseases.