A lariat-derived circular RNA is required for plant development in Arabidopsis
A lariat-derived circular RNA is required for plant development in Arabidopsis
复制标题
拟南芥植物发育需要源自套索的环状 RNA
DOI：
10.1007/s11427-017-9182-3
复制
发表时间：
2018-02-01
影响因子：
9.1
通讯作者：
Zheng, Binglian
中科院分区：
文献类型：
--
作者：
Cheng, Jinping;Zhang, Yong;Zheng, Binglian
套索RNA是在前mRNA剪接过程中产生的，传统上被认为是副产品，因为它在去分支后降解的过程中周转很快。然而，最近的发现发现，在高等真核生物中，许多套索RNA以环状形式积累。尽管套索来源的环状RNA(这里我们称之为laciRNAs)的显著积累和生物学后果在很大程度上仍不清楚。在这里，我们报道了来自AT5G37720的一个特定的laciRNA通过调节全球基因表达在植物发育中发挥重要作用。我们通过对拟南芥中17个在野生型植物中积累的laciRNAs进行了循环RNA测序。为了确定这些laciRNAs的生物学功能，我们为每个laciRNA构建了一对转基因植物，其中带有和不带有内含子的局部基因在野生型植物中分别过表达。通过比较两类转基因植物的形态表型和转录图谱，我们发现，来自AT5G37720第一内含子的laciRNA的过度表达导致了多效性表型，包括卷曲和簇生的叶片，开花晚，育性降低，并伴随着大约800个基因的表达改变。我们的结果提供了另一个例子，即特定的植物环状RNA在生理条件下以类似于其他非编码RNA的方式调节基因表达。
Lariat RNA is produced during pre-mRNA splicing, and it is traditionally thought as by-products, due to the quick turnover by debranching followed by degradation. However, recent findings identified many lariat RNAs accumulate with a circular form in higher eukaryotes. Although the remarkable accumulation, biological consequence of lariat-derived circular RNAs (here we name laciRNAs) remains largely unknown. Here, we report that a specific laciRNA from At5g37720 plays an essential role in plant development by regulating gene expression globally. We focus on 17 laciRNAs with accumulation in wild type plants by circular RNA sequencing in Arabidopsis. To determine biological functions of these laciRNAs, we constructed one pair of transgenic plants for each laciRNA, in which the local gene with or without introns was over-expressed in wild type plants, respectively. By comparing morphological phenotypes and transcriptomic profiles between two classes of transgenic plants, we show that over-expression of the laciRNA derived from the 1st intron of At5g37720 causes pleiotropic phenotypes, including curly and clustered leaf, late flowering, reduced fertility, and accompanied with altered expression of approximately 800 genes. Our results provide another example that a specific plant circular RNA regulates gene expression in a similar manner to that of other non-coding RNAs under physiological conditions.