N‐cadherin localization in taste buds of mouse circumvallate papillae
N‐cadherin localization in taste buds of mouse circumvallate papillae
复制标题
N-钙粘蛋白在小鼠外周乳头味蕾中的定位
DOI：
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Mami Kurumata
中科院分区：
文献类型：
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作者：
Rio Ikuta;Kanae Myoenzono;Jun Wasano;K. Hamaguchi;S. Hamada;Mami Kurumata
味蕾是味觉的感受器官，含有50-100个味蕾细胞。虽然这些细胞经历连续的更新，但味蕾的结构和功能完整性得以维持。味蕾和传入纤维之间突触连接形成和维持的分子机制仍然不明确。在本研究中，我们研究了N-cadherin在小鼠轮廓乳头味蕾中的定位，因为N-cadherin是经典的钙粘蛋白之一，对突触的形成和维持很重要。在光学显微镜水平，N‐钙粘蛋白主要在II型细胞和轮廓乳头内和周围结缔组织中的神经纤维中检测到。在超微结构水平，N-钙粘蛋白免疫反应性出现沿着细胞膜和在II型细胞的细胞内囊泡。N-钙粘蛋白免疫反应性在N-钙粘蛋白阳性II型细胞接触部位的传入终末膜中也很明显。在II型细胞和神经纤维之间的通道型突触中，N-钙粘蛋白存在于假定的神经递质释放区周围，但不在其内，通过与味觉细胞并列的大线粒体识别。目前的结果表明，N-钙粘蛋白是重要的形成或维持II型细胞传入突触的味蕾。
Taste buds, the receptor organs for taste, contain 50–100 taste bud cells. Although these cells undergo continuous turnover, the structural and functional integrity of taste buds is maintained. The molecular mechanisms by which synaptic connectivity between taste buds and afferent fibers is formed and maintained remain ambiguous. In the present study, we examined the localization of N‐cadherin in the taste buds of the mouse circumvallate papillae because N‐cadherin, one of the classical cadherins, is important for the formation and maintenance of synapses. At the light microscopic level, N‐cadherin was predominantly detected in type II cells and nerve fibers in the connective tissues in and around the vallate papillae. At the ultrastructural level, N‐cadherin immunoreactivity appears along the cell membrane and in the intracellular vesicles of type II cells. N‐cadherin immunoreactivity also is evident in the membranes of afferent terminals at the contact sites to N‐cadherin‐positive type II cells. At channel type synapses between type II cells and nerve fibers, N‐cadherin is present surrounding, but not within, the presumed neurotransmitter release zone, identified by large mitochondria apposed to the taste cells. The present results suggest that N‐cadherin is important for the formation or maintenance of type II cell afferent synapses in taste buds.
钙依赖性粘附对于前体的维持是必要的。
DOI：
10.1006/dbio.2001.0182
发表时间：
2001
期刊：
Developmental biology.
影响因子：
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作者：
McCarthy,M;Na,E;Neyt,C;Langston,A;Fishell,G
通讯作者：
Fishell,G
通过 N- 或 E-钙粘蛋白的心脏特异性表达来挽救 N-钙粘蛋白敲除。
DOI：
10.1242/dev.128.4.459
发表时间：
2001
期刊：
Development (Cambridge, England)
影响因子：
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作者：
Luo,Y;Ferreira-Cornwell,M;Baldwin,H;Kostetskii,I;Lenox,J;Lieberman,M;Radice,G
通讯作者：
Radice,G
小鼠 R-钙粘蛋白的细胞结合特异性和该基因的染色体定位。
影响因子：
4
作者：
Matsunami,H;Miyatani,S;Inoue,T;Copeland,NG;Gilbert,DJ;Jenkins,NA;Takeichi,M
通讯作者：
Takeichi,M