Simultaneous bioimaging of MMP-2 and MMP-7 via Au-Se constructed fluorescence nanoprobe
Simultaneous bioimaging of MMP-2 and MMP-7 via Au-Se constructed fluorescence nanoprobe
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通过 Au-Se 构建的荧光纳米探针同时对 MMP-2 和 MMP-7 进行生物成像
DOI：
10.1007/s11426-019-9646-5
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Tang Bo
中科院分区：
文献类型：
--
作者：
Gao Xiaonan;Guo Wenfei;Jiang Lulu;Hu Bo;Liu Xiaojun;Xu Kehua;Tang Bo
Au-Se bond strategy has been applied to construct fluorescence nanoprobe that is being used to simultaneously dual-image the tumor markers matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-7 (MMP-7)in vitro. The two Se-terminating ligand peptide chains respectively labeled with fluorescein isothiocyanate (FITC) and 5-Carboxytetramethylrhodamine (5-TAMRA) dyes are attached to the surface of Au nanoparticles (NPs). The nanoprobe can be specifically cut off by MMP-2 and MMP-7 respectively to carry out significant enhanced fluorescence recovery for simultaneously imaging both markers. The nanoprobe not only displays high selectivity and sensitivity towards MMP-2 and MMP-7, but also has strong anti-interference stability against biothiols and enhanced fidelity for avoiding ‘false positive’ results. This approach offers new perspectives in designing tumor-related early diagnosis approaches and also provides strategies for clinical applications.
