Cartilage progenitor cells combined with PHBV in cartilage tissue engineering
Cartilage progenitor cells combined with PHBV in cartilage tissue engineering
复制标题
软骨祖细胞联合PHBV在软骨组织工程中的应用
DOI：
10.1186/s12967-019-1855-x
复制
发表时间：
2019-03-29
影响因子：
7.4
通讯作者：
Liu, Kai
中科院分区：
文献类型：
--
作者：
Xue, Ke;Zhang, Xiaodie;Liu, Kai
BackgroundBone marrow-derived stem cells (BMSCs) and chondrocytes have been reported to present dedifferentiation and phenotypic loss during the chondrogenic differentiation process in cartilage tissue engineering, and cartilage progenitor cells (CPCs) are novel seeding cells for cartilage tissue engineering. In our previous study, cartilage progenitor cells from different subtypes of cartilage tissue were isolated and identified in vitro, but the study on in vivo chondrogenic characteristics of cartilage progenitor cells remained rarely. In the current study, we explored the feasibility of combining cartilage progenitor cells with poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) to produce tissue-engineered cartilage and compared the proliferation ability and chondrogenic characteristics of cartilage progenitor cells with those of bone marrow-derived stem cells and chondrocytes.MethodsThese three cells combined with PHBV were cultured in vitro for 1week without chondrogenic induction and then transplanted subcutaneously into nude mice for 6weeks. The cell-PHBV constructs were evaluated by gross observation, histological staining, glycosaminoglycan content measurement, biomechanical analysis and RT-PCR.ResultsThe chondrocyte-PHBV constructs and CPC-PHBV constructs became an ivory-whitish cartilage-like tissue, while the BMSC-PHBV constructs became vascularized 6weeks after the subcutaneous implantation. Histological examination showed that many typical cartilage structures were present in the chondrocyte group, some typical cartilage structures were observed in the CPC group, while no typical cartilage structures were observed in the BMSC group.ConclusionsCartilage progenitor cells may undergo chondrogenesis without chondrogenic induction and are better at chondrogenesis than BMSCs but worse than chondrocytes in the application of cartilage tissue engineering.
