Model connective tissue system: The effect of proteoglycan on the diffusional behavior of small non‐electrolytes and microions
Model connective tissue system: The effect of proteoglycan on the diffusional behavior of small non‐electrolytes and microions
复制标题
结缔组织系统模型：蛋白多糖对小非电解质和微离子扩散行为的影响
作者：
B. Preston;J. M. Snowden
作为结缔组织模型，我们研究了低分子量溶质在含聚阴离子材料的凝胶膜中的扩散行为。采用稳态同位素通量测量。结果表明，在明胶-琼脂糖基质中存在的“天然”蛋白聚糖溶液对非电解质的扩散影响可以忽略不计，但对离子种类的行为有显著影响。从多南平衡的角度讨论了反离子和离子的渗透率。显然，根据多南效应，并非多离子盐的所有反离子都可以看作是可移动的。Katchalsky等人的聚电解质理论被应用于凝胶膜的行为来解释这一观察结果。这些定量解释表明，蛋白质聚糖发生了反离子固定。
The diffusional behavior of low‐molecular‐weight solutes through gel membranes containing polyanionic material has been studied as a model for connective tissue. A steady‐state isotopic flux measurement is used. Results indicate that the presence of solutions of “native” proteoglycans within a gelatin‐agarose matrix has negligible effects on the diffusion of non‐electrolytes but markedly affects the behavior of ionic species. The permeabilities of counter‐and coions are discussed in terms of a Donnan equilibrium. It is evident that not all the counterions of the polyionic salt can be regarded as mobile in terms of the Donnan effect. The polyelectrolyte theories of Katchalsky et al. are applied to the behavior of the gel membranes to explain this observation. These quantitative interpretations suggest that counterion fixation by the proteoglycans occurs.