EPITHELIAL MOUSE MAMMARY CELL-LINE EXHIBITING NORMAL MORPHOGENESIS INVIVO AND FUNCTIONAL-DIFFERENTIATION INVITRO
EPITHELIAL MOUSE MAMMARY CELL-LINE EXHIBITING NORMAL MORPHOGENESIS INVIVO AND FUNCTIONAL-DIFFERENTIATION INVITRO
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DOI：
10.1073/pnas.81.12.3756
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发表时间：
1984-01-01
期刊：
影响因子：
--
通讯作者：
MEDINA, D
中科院分区：
文献类型：
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作者：
DANIELSON, KG;OBORN, CJ;MEDINA, D
从妊娠中期BALB/c小鼠的乳腺组织中分离出一株上皮细胞系，命名为COMA-1D。该细胞系在连续培养12个月后，表现出正常乳腺上皮细胞特有的几个特征，包括在体外诱导酪蛋白合成和清除同基因小鼠乳腺脂肪垫上的正常导管形态。在高密度培养中，细胞也形成穹顶，间接免疫荧光显示角蛋白中间丝阳性。COMA-1D细胞的染色体数目接近二倍体，不会在悬浮培养中生长，也不会在同基因宿主中产生肿瘤。该细胞系可用于研究乳腺细胞正常细胞分化的调控，以及上皮细胞向癌前表型和肿瘤表型的转化。
An epithelial cell line, designated COMMA-1D, was derived from mammary tissue of BALB/c mice in the middle of pregnancy. This line, in continuous cell culture for 12 mo., exhibits several characteristics distinctive of normal mammary epithelial cells, including induction of casein synthesis in vitro and normal duct morphogenesis in the cleared mammary fat pads of syngeneic mice. The cells also form domes in high density culture and are positive for keratin intermediate filaments by indirect immunofluorescence. COMMA-1D cells have a near diploid number of chromosomes and do not grow in suspension culture or produce tumors in syngeneic hosts. This cell line should prove useful for studies examining the regulation of normal cellular differentiation of mammary cells as well as transformation of epithelial cells to the preneoplastic and neoplastic phenotypes.