Membrane electroporation: The absolute rate equation and nanosecond time scale pore creation
Membrane electroporation: The absolute rate equation and nanosecond time scale pore creation
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DOI：
10.1103/physreve.74.021904
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发表时间：
2006-08-01
影响因子：
2.4
通讯作者：
Weaver, James C.
中科院分区：
文献类型：
--
作者：
Vasilkoski, Zlatko;Esser, Axel T.;Weaver, James C.
最近在生物系统中应用纳秒、兆伏特/米的电场脉冲显示了显著的细胞和亚细胞电场诱导效应，并重新引起了人们对电穿孔生物物理机制的兴趣。我们首先证明了基于实验参数输入的绝对速率理论与纳秒时间尺度上的膜孔生成是一致的。其次，我们使用一个基于Smoluchowski方程的模型来形成一个自洽的理论方法。对平面细胞膜贴片在10 ns上升和下降时间为1.5 ns的梯形脉冲作用下进行了分析。结果表明，可逆超电穿孔行为表现为跨膜电压、孔密度、膜电导、分水面积、孔分布和平均孔半径。我们进一步使用Krassowska的渐近电穿孔模型来分析纳秒脉冲，表明气孔的产生主导了电学响应，并且气孔膨胀在这个时间尺度上的影响可以忽略不计。
The recent applications of nanosecond, megavolt-per-meter electric field pulses to biological systems show striking cellular and subcellular electric field induced effects and revive the interest in the biophysical mechanism of electroporation. We first show that the absolute rate theory, with experimentally based parameter input, is consistent with membrane pore creation on a nanosecond time scale. Secondly we use a Smoluchowski equation-based model to formulate a self-consistent theoretical approach. The analysis is carried out for a planar cell membrane patch exposed to a 10 ns trapezoidal pulse with 1.5 ns rise and fall times. Results demonstrate reversible supraelectroporation behavior in terms of transmembrane voltage, pore density, membrane conductance, fractional aqueous area, pore distribution, and average pore radius. We further motivate and justify the use of Krassowska's asymptotic electroporation model for analyzing nanosecond pulses, showing that pore creation dominates the electrical response and that pore expansion is a negligible effect on this time scale.