Optimization of phototrophic hydrogen production by Rhodopseudomonas palustris PBUM001 via statistical experimental design
Optimization of phototrophic hydrogen production by Rhodopseudomonas palustris PBUM001 via statistical experimental design
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DOI：
10.1016/j.ijhydene.2009.05.116
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发表时间：
2009-09-01
影响因子：
7.2
通讯作者：
Vikineswary, S.
中科院分区：
文献类型：
--
作者：
Jamil, Zadariana;Annuar, Mohamad Suffian Mohamad;Vikineswary, S.
使用响应面法 (RSM) 优化了棕榈油厂废水 (POME) 中本土紫色非硫细菌沼泽红假单胞菌 PBUM001 的光养氢生产。研究的工艺参数包括接种量 (% v/v)、POME 浓度 (% v/v)、光强度 (klux)、搅拌 (rpm) 和 pH。使用响应面回归分析将累积产氢量和 COD 降低的实验数据拟合到二次多项式模型中。通过分析响应面三维曲面图和等高线图来确定最佳工艺条件的路径。对 Box-Behnken 设计收集的实验数据进行统计分析表明，POME 浓度为 100%（v/v）、接种量为 10%（v/v）、光照强度为 4.0 klux、搅拌速率为 250 rpm、pH 为 6 是最佳条件。在这些条件下获得的最大预测累积产氢量和 COD 降低量分别为 1.05 ml H-2/ml POME 和 31.71%。随后在最佳工艺值下进行的验证实验给出了累积氢的最大产量为 0.66 +/- 0.07 ml H-2/ml POME，COD 降低率为 30.54 +/- 9.85%。 (C) 2009 年国际氢能协会。由爱思唯尔有限公司出版。保留所有权利。
Phototrophic hydrogen production by indigenous purple non-sulfur bacteria, Rhodopseudomonas palustris PBUM001 from palm oil mill effluent (POME) was optimized using response surface methodology (RSM). The process parameters studied include inoculum sizes (% v/v), POME concentration (% v/v), light intensity (klux), agitation (rpm) and pH. The experimental data on cumulative hydrogen production and COD reduction were fitted into a quadratic polynomial model using response surface regression analysis. The path to optimal process conditions was determined by analyzing response surface three-dimensional surface plot and contour plot. Statistical analysis on experimental data collected following Box-Behnken design showed that 100% (v/v) POME concentration, 10% (v/v) inoculum size, light intensity at 4.0 klux, agitation rate at 250 rpm and pH of 6 were the best conditions. The maximum predicted cumulative hydrogen production and COD reduction obtained under these conditions was 1.05 ml H-2/ml POME and 31.71% respectively. Subsequent verification experiments at optimal process values gave the maximum yield of cumulative hydrogen at 0.66 +/- 0.07 ml H-2/ml POME and COD reduction at 30.54 +/- 9.85%. (C) 2009 International Association for Hydrogen Energy. Published by Elsevier Ltd. All rights reserved.