The contribution of mitochondrial respiratory complexes to the production of reactive oxygen species
The contribution of mitochondrial respiratory complexes to the production of reactive oxygen species
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DOI：
10.1023/a:1005507913372
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发表时间：
2000-04-01
影响因子：
3
通讯作者：
Degli Esposti, M
中科院分区：
文献类型：
--
作者：
McLennan, HR;Degli Esposti, M
本工作的重点是区分线粒体氧化还原复合物的贡献，在细胞呼吸过程中的活性氧（ROS）的生产。我们能够准确地测量，第一次，基础生产的ROS在未偶联的条件下，通过使用一个非常敏感的方法，基于荧光探针二氯二氢荧光素二乙酸酯。该方法还能够检测线粒体制剂中内源性底物氧化产生的ROS，并可应用于线粒体和活细胞。与普遍接受的复合物III（泛醇：细胞色素c还原酶）是线粒体ROS产生的主要贡献者的观点相反，我们发现复合物I（NADH-泛醌还原酶）和复合物I（琥珀酸-泛醌还原酶）是在非偶联条件下长时间呼吸过程中ROS的主要产生者。特别是复合物II似乎有助于细胞中ROS的基础产生。
This work was focused on distinguishing the contribution of mitochondrial redox complexes to the production of reactive oxygen species (ROS) during cellular respiration. We were able to accurately measure, for the first time, the basal production of ROS under uncoupled conditions by using a very sensitive method, based on the fluorescent probe dichlorodihydrofluorescein diacetate. The method also enabled the detection of the ROS generated by the oxidation of the endogenous substrates in the mitochondrial preparations and could be applied to both mitochondria and live cells. Contrary to the commonly accepted view that complex III (ubiquinol:cytochrome c reductase) is the major contributor to mitochondrial ROS production, we found that complex I (NADH-ubiquinone reductase) and complex It (succinate-ubiquinone reductase) are the predominant generators of ROS during prolonged respiration under uncoupled conditions. Complex II, in particular, appears to contribute to the basal production of ROS in cells.