Different dimethylsulphoniopropionate-producing ability of dinoflagellates could affect the structure of their associated bacterial community
Different dimethylsulphoniopropionate-producing ability of dinoflagellates could affect the structure of their associated bacterial community
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DOI：
10.1016/j.algal.2021.102359
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发表时间：
2021-07
期刊：
影响因子：
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通讯作者：
Qun Lin;Linxin Shang;Xiaoli Wang;Zhong Hu;Hong Du;Hui Wang
中科院分区：
文献类型：
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作者：
Qun Lin;Linxin Shang;Xiaoli Wang;Zhong Hu;Hong Du;Hui Wang
甲藻在海洋生态系统中对二甲基磺基丙酸盐（DMSP）的产生起着重要作用。甲藻DMSP是异养浮游细菌重要的还原性硫源和碳源。尽管甲藻具有出色的DMSP生物合成能力，并且不同物种和菌株细胞DMSP浓度的种间差异已得到很好的描述，但DMSP水平对微生物群落结构的影响仍不清楚。研究了7种甲藻合成DMSP的能力及其对相关细菌群落组成的影响。网状原角藻的DMSP总浓度（2397.25 ± 187.17 μM）和单细胞DMSP含量（34.25 ± 2.67 pmol cell−1）最高，而前甲藻和前沟藻的DMSP含量显著低于其他甲藻。甲藻培养过程中细菌群落结构的测定采用了培养依赖型和非培养依赖型两种方法。不同DMSP含量的甲藻培养物中细菌的丰富度和多样性存在显著差异。此外，在6种甲藻的培养物中发现了丰富的玫瑰杆菌分支细菌（众所周知的DMSP降解菌）。基因组分析表明，从甲藻中分离的玫瑰杆菌分支细菌可以通过裂解途径降解DMSP。结果表明，DMSP可能是影响甲藻藻圈细菌群落丰度和组成的重要因素。
Dinoflagellate plays an important role in the production of dimethylsulphoniopropionate (DMSP) in marine ecosystem. DMSP derived from dinoflagellates is an important reducing sulfur and carbon source for heterotrophic bacterioplankton. Although dinoflagellates have outstanding ability in DMSP biosynthesis, and the inter-specific variation of cellular DMSP concentrations in different species and strains has been well described, the effects of DMSP level on microbial community structure are still unclear. In this study, the ability of seven dinoflagellates to synthesize DMSP and their effects on the composition of associated bacterial community were investigated. Total concentration of DMSP (2397.25 ± 187.17 μM) and single cell DMSP content (34.25 ± 2.67 pmol cell−1) were the highest inProtoceratium reticulatum, while the DMSP contents of ProrocentrumtexanumandAmphidiniumcarteraewere significantly lower than those of other dinoflagellates. Bacterial community structure in dinoflagellate culture was determined by cultivation-dependent and cultivation-independent approaches. There were significantly differences in the richness and diversity of bacteria in different dinoflagellate cultures with different DMSP contents. In addition, abundant of Roseobacter-clade bacteria (well-known DMSP degraders) were found in the cultures of six dinoflagellates. Genomic analysis indicated that theRoseobacter-clade bacteria isolated from dinoflagellate could degrade DMSP by cleavage pathway. The results demonstrated that DMSP could be an important factor affecting the abundance and composition of bacterial community in phycosphere of dinoflagellates.