Molecular analysis of DLA-DRBB1 polymorphism.
Molecular analysis of DLA-DRBB1 polymorphism.
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DLA-DRBB1 多态性的分子分析。
DOI：
10.1111/j.1399-0039.1996.tb02669.x
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Storb,R
中科院分区：
文献类型：
--
作者：
Wagner,JL;Burnett,RC;Works,JD;Storb,R
分析了犬主要组织相容性复合体II类DRB基因DRB1的多态性。通过聚合酶链反应（PCR）扩增编码多态性81结构域的外显子2。对250只犬的PCR产物进行了克隆和测序。鉴定出18个等位基因。大多数氨基酸组成的变化发生在肽结合位点的位置。这些序列的遗传表现为孟德尔分离，每只狗有一个或两个等位基因。聚类分析的核苷酸和预测的氨基酸序列细分为三个主要的等位基因组的犬DRBB1等位基因。非同义变化的数量高于指示阳性选择的推定抗原识别位点中的同义变化的数量。所产生的数据可以作为开发犬DRBB1基因分型试验的基础。
The polymorphism of the canine major histocompatibility complex class II DRB gene, DRBB1, was analyzed. Exon 2 that encodes the polymorphic 81 domain was amplified by the polymerase chain reaction (PCR). The PCR product from 250 dogs was cloned and sequenced. Eighteen alleles were identified. Most of the variation in amino acid composition occurred at positions in the peptide binding site. Inheritance of these sequences showed Mendelian segregation with one or two alleles per dog. Cluster analysis of the nucleotide and predicted amino acid sequences subdivided the canine DRBB1 alleles into three major allelic groups. The number of nonsynonymous changes was higher than the number of synonymous changes in the putative antigen recognition sites indicative of positive selection. The data generated can serve as a basis for developing a typing assay for the canine DRBB1 gene.